Primer group for detecting BMPR1B gene mutation, purpose of primer group and kit containing primer group
A technology of primer set and kit, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, biochemical equipment and methods, etc., can solve problems such as unreachable detection, ensure accuracy and uniqueness, and have a wide range of applications , the effect of improving the quality of the population
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Embodiment 1
[0030] According to the 1st to 10th exon region, 3'UTR, 5'UTR and promoter sequence of the BMPR1B gene published by NCBI Gene Bank (NG_009245), a primer set for detecting whether the BMPR1B gene is mutated was designed. The set includes a PCR amplification primer set and a PCR sequencing primer set corresponding to the above regions, and the results are shown in Table 1 and Table 2.
Embodiment 2
[0032] A kit for detecting BMPR1B gene mutations, including PCR amplification primer sets and PCR sequencing primer sets corresponding to the 1st to 10th exon regions, 3'UTR, 5'UTR and promoter sequences of the BMPR1B gene, a total of 24 each For the primers 1OD, refer to Table 1 and Table 2 for the sequences of each pair of primers.
[0033] This kit is stored at -20°C, and repeated freezing and thawing should be avoided as much as possible.
Embodiment 3
[0035] Kits for detecting BMPR1B gene mutations, including:
[0036] (1) PCR amplification primer set and PCR sequencing primer set for the 1st to 10th exon region, 3'UTR, 5'UTR and promoter sequence of BMPR1B gene, a total of 24 pairs, each primer 1OD, each pair of primer sequences See Tables 1 and 2.
[0037] (2) PCR amplification reagent: 2.5mM dNTP mixture 40μl, 5×amplification buffer (containing Mg 2+ ) 100μl, 5Units / ul Taq DNA polymerase 5μl.
[0038] (3) PCR product purification reagents: 1 U / ul SAP enzyme 20 μl, 10 U / ul ExoI enzyme 10 μl.
[0039] (4) PCR sequencing reagents: BigDye 3.1 mix 50μl, 0.125M EDTA solution 50μl, absolute ethanol 1ml, 75% ethanol solution 1.5mL, HIDI solution 500μl.
[0040] This kit is stored at -20°C, and repeated freezing and thawing should be avoided as much as possible.
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