A kind of preparation method of environment-friendly organic waste water biological purification agent
A technology for biological purifiers and organic wastewater, which is applied in biochemical equipment and methods, microorganism-based methods, chemical instruments and methods, etc., can solve the problems of lack of selectivity, high treatment cost, low efficiency in the treatment process, etc. The process is simple and easy to implement, the reuse rate is high, and the production cycle is short.
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Embodiment 1
[0036] Embodiment 1: the influence of sodium alginate dosage on immobilization effect
[0037] Prepare the immobilization matrix of sodium alginate and biochar, the ratio of sodium alginate to immobilization matrix (m:V) is 0.5, 1.0, 2.0, 3.0 and 5.0 g:100.0 mL, the ratio of biochar to immobilization matrix (m:V) is 0.2 g:100.0 mL. Add the wet Aspergillus niger cultured for 30 h to the immobilization matrix, so that the ratio (m:V) of the wet Aspergillus niger to the immobilization matrix is 6.0 g:100.0 mL, mix the above solution evenly, and perform the immobilization operation , at a height of about 10 cm from the liquid surface, use a ball maker with a diameter of 4 mm to drop the mixed solution into 20.0 g / L calcium chloride solution drop by drop, immobilize for 2 hours, filter out the balls, and use Wash 2-3 times with sterile water, collect and set aside.
[0038] The immobilized Aspergillus niger was added to the deltamethrin wastewater with a concentration of 50.0 m...
Embodiment 2
[0039] Embodiment 2: the influence of calcium chloride concentration on immobilization effect
[0040]Using the determined optimal conditions and under the condition that other conditions remain unchanged, drop the mixed solution into calcium chloride solutions with mass concentrations of 5.0, 10.0, 20.0, 30.0 and 50.0 g / L respectively. After 2 hours, the pellets were filtered out, washed 2-3 times with sterile water, collected and set aside.
[0041] The experimental methods and evaluation indicators are the same as above.
Embodiment 3
[0042] Embodiment 3: The influence of biochar dosage on immobilization effect
[0043] Using the determined optimal conditions and keeping other conditions unchanged, the effect of the amount of biochar on the performance of immobilized Aspergillus niger was studied, and the ratio (m:V) of biochar to immobilized substrate was set to 0.01 and 0.05 respectively , 0.10, 0.20 and 0.50 g: 100.0 mL, the preparation method is the same as above.
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