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Compound microbial liquid for treating anaerobic digested sludge and novel bioleaching method

A composite microbial bacteria and anaerobic digestion technology, which is applied in the field of processing anaerobic digested sludge composite microbial bacteria liquid and biological leaching, can solve the problems of rising and the deterioration of sludge dewatering effect, and achieves a simple process and reduces the actual operation operation. Difficulty, the effect of reducing the operating cost of bioleaching

Active Publication Date: 2018-05-29
南京贝克特环保科技有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0008] Aiming at the phenomenon that the sludge specific resistance and pH increase instead of falling when the existing traditional bioleaching method directly treats anaerobic digested sludge, which leads to the deterioration of the sludge dehydration effect, the present invention provides a bioleaching method for anaerobically digested sludge. The new leaching method uses a composite bacterial solution composed of Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella ZW2, and Pichia pastoris D13 to carry out bioleaching of anaerobic digested sludge

Method used

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  • Compound microbial liquid for treating anaerobic digested sludge and novel bioleaching method

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Effect test

Embodiment 1

[0047] Bioleaching test of anaerobic digested sludge from a sewage treatment plant in Nanjing

[0048] According to the technique mentioned in the present invention, according to flow process (see attached figure 1 ) to treat the anaerobic digestion sludge of the sewage treatment plant. Take about 20L of anaerobic digested sludge from the plant. The basic properties of the sludge are as follows: pH value is 7.10, solid content is 4.06%, organic matter is 48.9%, and sludge specific resistance is 6.8×10 13 m / kg, total nitrogen 35.1%, total phosphorus 27%.

[0049] (1) Using anaerobic digestion sludge to cultivate complex microbial flora

[0050] Thiobacillus ferrooxidans LX5 (A.ferrooxidans LX5, preservation number is CGMCC NO.0727), Thiobacillus thiooxidans TS6 (A.thiooxidans TS6, preservation number CGMCC NO.0759), Bordetella sp. ZW2 (Bordetella sp .preservation number CGMCC NO.3033), Pichia pastoris D13 (P.spartinae D13, preservation number CGMCC NO.3035) were inoculated i...

Embodiment 2

[0068] Bioleaching test of anaerobic digested sludge from a sewage treatment plant in Wuxi

[0069] According to the technique mentioned in the present invention, according to flow process (see attached figure 1 ) to treat the anaerobic digestion sludge of the sewage treatment plant. Take about 100L of anaerobic digested sludge from the plant. The basic properties of the sludge are as follows: pH value is 7.56, solid content is 3.0%, organic matter is 50.1%, and the specific resistance of sludge is 8.37×10 13 m / kg, total nitrogen 35.1%, total phosphorus 27%.

[0070] (1) Using anaerobic digestion sludge to cultivate complex microbial flora

[0071] Inoculate Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella ZW2, and Pichia pastoris D13 into their respective culture media (Thiobacillus ferrooxidans LX5 medium g / L: (NH 4 ) 2 SO 4 3.5, KCl 0.119, K 2 HPO 4 0.58, Ca(NO 3 ) 2 4H 2 O 0.168, MgSO 4 ·7H 2 O 4.42 with 10N H 2 SO 4 Adjust pH to 2.5;...

Embodiment 3

[0077] Bioleaching test of anaerobic digested sludge from a sewage treatment plant in Zhengzhou

[0078] According to the technique mentioned in the present invention, according to flow process (see attached figure 1 ) to treat the anaerobic digestion sludge of the sewage treatment plant. About 500L of anaerobic digested sludge from the plant was taken, and the basic properties of the sludge were determined as follows: the pH value is 7.32, the solid content is 3.6%, the organic matter is 48.6%, and the specific resistance of the sludge is 6.37×10 13 m / kg, total nitrogen 33.1%, total phosphorus 25%.

[0079] (1) Using anaerobic digestion sludge to cultivate complex microbial flora

[0080] Inoculate Thiobacillus ferrooxidans LX5, Thiobacillus thiooxidans TS6, Bordetella ZW2, and Pichia pastoris D13 into their respective culture media (Thiobacillus ferrooxidans LX5 medium g / L: (NH 4 ) 2 SO 4 3.5, KCl 0.119, K 2 HPO 4 0.58, Ca(NO 3 ) 2 4H 2 O 0.168, MgSO 4 ·7H 2 O ...

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Abstract

The invention discloses a bioleaching method for anaerobic digested sludge and belongs to the technical field of sludge treatment. Bioleaching work flora is compounded by introducing new microbial strains, continuous domestication is performed, an inoculum obtained through domestication is mixed with the anaerobic digested sludge for bioleaching treatment, the new microbial strains are used for degrading small-molecular organic acids and secreting surface active materials to rapidly remove EPSs (extracellular polymeric substances) wrapping surfaces of sludge cells, bioleaching reaction time isshortened to 12-24 h, the sludge obtained after bioleaching directly enters a plate-and-frame filter press for dehydration, and a mud cake with water content lower than 60% is obtained. The method adopts simple process, no chemical reagent is needed due to complete dependence on action of microbes, and the method has the advantages of high dehydration efficiency, low operation cost and low technological operation difficulty.

Description

technical field [0001] The invention belongs to the technical field of sludge treatment, and more specifically relates to a novel method for treating complex microbial liquid and biological leaching of anaerobic digested sludge. Background technique [0002] In recent years, with the acceleration of my country's urbanization process and the improvement of environmental quality standards, the amount of sewage to be treated has increased sharply. Therefore, various sewage treatment processes such as oxidation ditch, biological filter and oxidation pond have been widely used at this stage, and finally Lead to a large amount of another pollutant (sludge) in sewage treatment. The composition of sludge is very complex, and the composition of sludge from different sources varies greatly. Sludge mainly includes bacteria micelles formed by various microorganisms and a collection of organic and inorganic substances adsorbed. Only timely and effective treatment of sludge and Disposal c...

Claims

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Application Information

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IPC IPC(8): C02F11/04C02F11/12C12N1/20C12N1/16C12N1/36C12R1/84C12R1/01
CPCC02F11/04C02F11/122C12N1/16C12N1/20C12N1/36
Inventor 周立祥李浙英颜成
Owner 南京贝克特环保科技有限公司
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