Sucrose invertase industrial production method

Abstract

The invention discloses a sucrose invertase industrial production method which comprises the following steps: S1, preparing a primary seed culture solution; S2, preparing a secondary seed culture solution; S3, performing seed tank liquid culture; S4, performing fermentation tank liquid culture; S5, separating out wet thallus after the end of the fermentation culture; S6, preparing into a bacteriumsuspension; S7, cyclically crushing by using a high-pressure homogenizer; S8, performing graded filtering treatment on crushed liquid through a hollow fiber membrane, a hollow fiber microfiltration membrane of 0.2 mum, a hollow fiber membrane of 300,000 Dalton and a hollow fiber membrane of 30,000 Dalton to obtain a high-purity sucrose invertase concentrate; S9, performing sterile filtration on the sucrose invertase concentrate through a liquid pre-filter of 0.5 mum and a liquid sterilizing filter of 0.22 mum to obtain a sterile enzyme solution; S10, performing precision pharmaceutical low temperature spray drying on the sterile enzyme solution to obtain a sterile sucrose invertase powder. The sucrose invertase industrial production method disclosed by the invention is high in product yield, high in activity, high in purity and controllable in production cost and conforms to food enzyme preparation quality requirements.

Description

technical field [0001] The invention relates to the technical field of food deep processing, in particular to an industrial production method of sucrose invertase. Background technique [0002] Sucrose is a commonly used sweetener in the field of food processing, but it takes a long time to dissolve during use, which limits its application to a certain extent. Sucrose invertase can hydrolyze sucrose to produce fructose and glucose, and the mixture is called invert syrup. Inverted syrup has the advantages of fast dissolution, low crystallization and good taste, so it is widely used in food processing industries such as beverages and baking. [0003] Sucrose is a renewable carbohydrate produced by photosynthesis in plants (sugar cane and sugar beet), with global production exceeding 100 million tons per year. Sucrose is hydrolyzed by sucrose invertase to obtain glucose and fructose, and monosaccharides enter the glycolytic fermentation pathway in microbial cells to decompose...

AI Technical Summary

Problems solved by technology

[0004] In recent years, with the improvement of people's living standards and the emphasis on renewable resources, sucrose invertase has received more and more attention, but it has not been widely used in China, because the current commercial sucrose invertase The purity is not high and the price is expensive, which limits its application in the field of food processing and the field of renewable resources