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Microbial flocculating agent for phosphorus removal, and preparation method and application thereof

A microbial flocculant and floc-producing bacteria technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost of chemical agents and limited application, and achieve low cost, good phosphorus removal effect, The effect of improving raw material utilization and production efficiency

Inactive Publication Date: 2018-05-22
卢梅雅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest feature of the chemical phosphorus removal system is that the phosphorus removal effect can be controlled by controlling the dosage of chemicals, but the cost of chemical agents is usually high, which limits its application in practical projects

Method used

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  • Microbial flocculating agent for phosphorus removal, and preparation method and application thereof
  • Microbial flocculating agent for phosphorus removal, and preparation method and application thereof
  • Microbial flocculating agent for phosphorus removal, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of a kind of microbial flocculant of the present embodiment comprises:

[0044] 1. Activated bacteria

[0045] (1) Inoculate Mucor genevensis CGMCC 3.4901 in sterilized bran medium and culture at 28°C for 5 days. The number of spores of Mucor genevensis in the culture exceeds 10 8 Individual / gram culture, get Geneva mucormycosis agent, stand-by.

[0046] (2) Inoculate Streptomyces pallidus CGMCC4.1384 in the sterilized Gaoshi No. 1 slant medium, cultivate it at 30°C for 7 days, and inject sterile water with glass beads into the test tube slant , after fully oscillating, filter into a single spore suspension, use photoelectric turbidimetry to detect the number of spores, and make the number of spores 10 9 individual / mL spore suspension for use.

[0047] (3) Inoculate Clostridium cellulolyticum (Clostridium cellulolyticum) ATCC35319 into a sterilized 250mL anaerobic bottle containing 100mL DCB-1 medium (containing 5.0g / L crushed corn stalks), and...

Embodiment 2

[0057] 1. Activated bacteria

[0058] (1) Inoculate Trichoderma reesei (Trichoderma reesei) CGMCC3.3711 in sterilized bran medium, and culture it at 28°C for 5 days, and the number of spores of Mucor Geneva in the culture exceeds 10 8 When individual / gram culture, obtain Trichoderma reesei fungal agent, stand-by.

[0059] (2) Inoculate Streptomyces spororaveus CGMCC4.5873 in the sterilized Gaoshi No. 1 slant medium, and after culturing at 30°C for 7 days, inject sterile glass beads with glass beads into the test tube slant. After fully shaking the water, filter it into a single spore suspension, use photoelectric turbidimetry to detect the number of spores, and make the number of spores 10 9 individual / mL spore suspension for use.

[0060] (3) Inoculate Clostridium cellulolyticum (Clostridium cellulolyticum) ATCC35319 into a sterilized 250mL anaerobic bottle containing 100mL DCB-1 medium (containing 5.0g / L crushed corn stalks), and culture at 35°C for 3 days , collect the b...

Embodiment 3

[0069] 1. Activated bacteria

[0070] (1) Inoculate Rhizopus oryzae (Rhizopus oryzae) CGMCC 3.5818 in sterilized bran medium and culture at 28°C for 5 days. The number of spores of Mucor Geneva in the culture exceeds 10 8 When individual / gram culture, obtain Trichoderma reesei inoculant, stand-by.

[0071] (2) Inoculate Streptomyces lateritius CGMCC4.1427 in the sterilized Gaoshi No. 1 slant medium, cultivate it at 30°C for 7 days, and inject sterile water with glass beads into the test tube slant , after fully oscillating, filter into a single spore suspension, use photoelectric turbidimetry to detect the number of spores, and make the number of spores 10 9 individual / mL spore suspension for use.

[0072] (3) Inoculate Clostridium cellulolyticum (Clostridium cellulolyticum) ATCC35319 into a sterilized 250mL anaerobic bottle containing 100mL DCB-1 medium (containing 5.0g / L crushed corn stalks), and culture at 35°C for 3 days , collect the bacteria, and make a concentration ...

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Abstract

The invention discloses a microbial flocculating agent for phosphorus removal, and a preparation method and an application thereof. The preparation method comprises the following steps: inoculating acrude fiber raw material with flocculant-producing bacteria, and carrying out aerobic fermentation to obtain a primary fermentation material; inoculating the primary fermentation material with Clostridium cellulolyticum, and carrying out anaerobic fermentation to obtain a secondary fermentation material; inoculating the secondary fermentation material with myxobacteria, and carrying out aerobic fermentation to obtain a tertiary fermentation material; and extracting the tertiary fermentation material with ethyl acetate to obtain the microbial flocculating agent. The the crude fiber raw materialis inoculated with the flocculant-producing bacteria, the flocculant-producing bacteria are used to perform primary biological conversion on the crude fiber raw material, the primary fermentation material is continuously inoculated with the Clostridium cellulolyticum, the secondary fermentation material is continuously inoculated with the myxobacteria, and the myxobacteria can use dead or low-activity flocculant-producing bacteria in the secondary fermentation material as a nutrient source and further generate metabolites capable of precipitating phosphates in sewage, so the raw material utilization rate and the production efficiency are greatly improved.

Description

technical field [0001] The invention relates to the technical field of phosphorus-rich sewage treatment, in particular to a phosphorus-removing microbial flocculant and its preparation method and application. Background technique [0002] At present, in the process of urban sewage treatment, not only COD and SS are required to be taken out, but also higher requirements are put forward for phosphorus removal and nitrogen removal. However, in rural areas, there are still many people who use phosphorus-rich washing powder because of weak environmental awareness and greed for cheap, which leads to the situation of phosphorus pollution in rural rivers is not optimistic. [0003] Sewage phosphorus removal technology can be divided into two categories: biological phosphorus removal and chemical phosphorus removal. Among them, the essence of biological phosphorus removal is to excessively absorb phosphorus in wastewater under aerobic conditions through phosphorus accumulating bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P1/04C02F3/34C02F101/10C12R1/145C12R1/01C12R1/785C12R1/885C12R1/66C12R1/80C12R1/845
CPCC02F3/34C02F3/347C02F2101/105C12P1/02C12P1/04C12N1/145C12N1/205C12R2001/01C12R2001/145C12R2001/66C12R2001/785C12R2001/80C12R2001/845C12R2001/885
Inventor 卢梅雅
Owner 卢梅雅
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