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Application of immobilized laccase in methylene blue decoloration

A technology of methylene blue decolorization and immobilization of laccase, which is applied to the direction of redox enzymes fixed on or in inorganic carriers, fixed on/in organic carriers, etc., and can solve the technical difficulties of chromaticity removal of organic matter and other problems , to achieve high reuse rate, increase surface area, and high-efficiency treatment

Inactive Publication Date: 2018-05-11
NORTH CHINA ELECTRIC POWER UNIV (BAODING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prominent problems in dye industry wastewater treatment include technical difficulties in the removal of chroma and mineralization of complex refractory organic matter

Method used

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  • Application of immobilized laccase in methylene blue decoloration
  • Application of immobilized laccase in methylene blue decoloration
  • Application of immobilized laccase in methylene blue decoloration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Weigh 0.2 g of the carboxyl carbon nanotubes obtained from the above reaction and disperse them into 5 mL of 6% n-octane solution, add 0.2 g of sodium dodecyl sulfate and 30 mL of water, and dissolve in ice for 15 min with ultrasound;

[0031] (2) Add 4 mL of methyl methacrylate, 1 mL of divinylbenzene, and 8 mL of 1% potassium persulfate at a reaction temperature of 70 °C, stir and polymerize for 24 h. After the reaction is completed, cool down to room temperature naturally and centrifuge;

[0032] (3) The solid obtained from the above reaction is alternately washed with water and ethanol, dried in vacuum, cooled, and the product is collected to obtain black solid particles, which are carboxyl carbon nanotubes coated with polymethyl methacrylate;

[0033] (4) Weigh 0.2 g of carboxyl carbon nanotubes coated with polymethyl methacrylate, add 20 ml of 10% glutaraldehyde aqueous solution, stir mechanically at room temperature for 6 h, and wash with water after the react...

Embodiment approach 2

[0038] (1) Weigh 0.2 g of the carboxyl carbon nanotubes obtained from the above reaction and disperse them into 5 mL of 6% n-octane solution, add 0.2 g of sodium dodecyl sulfate and 30 mL of water, and dissolve in ice for 15 min with ultrasound;

[0039] (2) Add 4 mL of methyl methacrylate, 1 mL of divinylbenzene, and 8 mL of 1% potassium persulfate at a reaction temperature of 70 °C, stir and polymerize for 24 h. After the reaction is completed, cool down to room temperature naturally and centrifuge;

[0040] (3) The solid obtained from the above reaction is alternately washed with water and ethanol, dried in vacuum, cooled, and the product is collected to obtain black solid particles, which are carboxyl carbon nanotubes coated with polymethyl methacrylate;

[0041] (4) Weigh 0.2 g of carboxyl carbon nanotubes coated with polymethyl methacrylate, add 20 ml of 10% glutaraldehyde aqueous solution, stir mechanically at room temperature for 6 h, and wash with water after the react...

Embodiment approach 3

[0046] (1) Weigh 0.2 g of the carboxyl carbon nanotubes obtained from the above reaction and disperse them into 5 mL of 6% n-octane solution, add 0.2 g of sodium dodecyl sulfate and 30 mL of water, and dissolve in ice for 15 min with ultrasound;

[0047] (2) Add 4 mL of methyl methacrylate, 1 mL of divinylbenzene, and 8 mL of 1% potassium persulfate at a reaction temperature of 70 °C, stir and polymerize for 24 h. After the reaction is completed, cool down to room temperature naturally and centrifuge;

[0048] (3) The solid obtained from the above reaction is alternately washed with water and ethanol, dried in vacuum, cooled, and the product is collected to obtain black solid particles, which are carboxyl carbon nanotubes coated with polymethyl methacrylate;

[0049] (4) Weigh 0.2 g of carboxyl carbon nanotubes coated with polymethyl methacrylate, add 20 ml of 10% glutaraldehyde aqueous solution, stir mechanically at room temperature for 6 h, and wash with water after the react...

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Abstract

The invention discloses immobilized laccase and an application of the immobilized laccase in methylene blue decoloration. The immobilized laccase takes a composite prepared through coating of the surface of a carboxylic carbon nanotube with polymethyl methacrylate as a carrier and glutaraldehyde as a binding agent, and laccase is immobilized on the carboxylic carbon nanotube coated with polymethylmethacrylate with a covalent binding method. The carrier adopted in the immobilized laccase is the carboxylic carbon nanotube coated with polymethyl methacrylate, so that the laccase load rate is high, the adsorption and decomposition functions can be played simultaneously to perform efficient decoloration on methylene blue, the decoloration rate can be up to 95%-97% and still exceeds 83% after the immobilized laccase is repeatedly used 10 times, and the immobilized laccase has the advantages of stable physical and chemical properties, high laccase load rate, high repeated utilization rate, efficient treatment and the like.

Description

technical field [0001] The invention relates to an application of immobilized laccase in the decolorization of methylene blue, which belongs to the field of water treatment in environmental protection. Background technique [0002] Laccase is a copper-containing polyphenol oxidase composed of peptide chains, sugars and Cu 2+ It consists of 3 parts. Laccase is widely distributed in plants, fungi, a few insects and bacteria in nature, and laccase has also been found in kidney and serum of some animals. Laccase can catalyze the oxidation reaction of many compounds with a wide range of substrates, including aminophenols, polyphenols, polyamines, lignin, aryldiamines, vanillic acid, veratrol, ferulic acid and Tween, etc. The reaction product is water, which is green and environmentally friendly. Therefore, laccase is widely used in various fields, such as: pulp and paper field, biosynthesis field, food field, energy field, wood processing field, environmental protection field...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08C02F3/34C02F101/38
CPCC02F3/342C02F2101/308C02F2101/40C12N9/0061C12N11/08C12N11/14C12Y110/03002
Inventor 张一梅赖毓娴武盼盼赵亚龙
Owner NORTH CHINA ELECTRIC POWER UNIV (BAODING)
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