A kind of purification method of guava leaf triterpenoids

Abstract

The invention discloses a purifying method of a psidium guajava Linn leaf triterpenoid. The purifying method comprises the following steps: (1) cleaning and drying psidium guajava Linn leaves, and smashing thepsidium guajava Linn leaves into powder; (2) extracting psidium guajava Linn leaf powder by utilizing an ultrasonic extraction method, and collecting an extracting solution; (3) carrying outvacuum filtration on an obtained triterpenoid crude extracting solution, and collecting filtrate; then concentrating the filtrate, collecting a concentrated solution, and filtering by using an organicmicroporous filter membrane, thus obtaining a triterpenes richment; (4) adsorbing triterpenes matters from the triterpenes richment by adopting macroporous adsorption resin, and eluting, thus a purified psidium guajava Linn leaf triterpenoid. According to the purifying method disclosed by the invention, a separation and purification method integrating the ultrasonic extraction method, macroporousadsorption resin purification and HPLC (High-Performance Liquid Chromatography) into a whole is established, and the purifying method has the advantages of low energy consumption, fast extracting speed, high yield and high purity; compared with a conventional solvent extracting method, the yield is increased by 60 percent or more, and the purity is increased from original 60 to 65 percent to 90 percent or more.

Description

technical field [0001] The invention belongs to the field of compound extraction, in particular to a method for purifying guava leaf triterpenoids. Background technique [0002] Guava (Psidium guajava Linn.) Myrtaceae tree, native to South America. Cultivated in various places in South China, wild species are common, and it reaches the Anning Valley in the southwestern part of Sichuan in the north. It grows in wasteland or low hills; the fruit is edible; the leaves contain volatile oil and tannin, etc., which are used medicinally to stop dysentery and stop bleeding. , invigorating the stomach and other effects; the leaves are boiled to remove tannin, dried for tea, sweet in taste, and has heat-clearing effect. Studies on the chemical composition of guava leaves show that: guava leaves contain proteins, polysaccharides, polyphenols, flavonoids and triterpenoids. Triterpenoids (triterpenoids) refer to terpenoids derived from squalene in terms of biological origin, usually co...

AI Technical Summary

Problems solved by technology

[0003] Among the few existing technologies, the extraction of triterpenoids generally adopts the traditional solvent extraction method. The traditional method takes a long time to extract, the process is cumbersome and the purity is low, which is difficult to meet the needs of modern development.

And at present, there is no extraction and purification method of guava leaf triterpenoids