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Application of Iron Ion in Inhibiting RNA Virus

A technology of RNA virus and iron ion, applied in the field of biomedical treatment

Active Publication Date: 2022-06-28
中国科学院上海免疫与感染研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no specific treatment for Zika virus, so there is an urgent need to develop specific anti-Zika virus drugs

Method used

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  • Application of Iron Ion in Inhibiting RNA Virus
  • Application of Iron Ion in Inhibiting RNA Virus
  • Application of Iron Ion in Inhibiting RNA Virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 Inhibitory effect of iron ions on influenza virus

[0104] method:

[0105] Ferric ammonium citrate (FAC), ferric citrate (FeCit) and ferrous ammonium sulfate (AIS) were added to the supernatant of human lung epithelial cell line A549 cells infected with influenza PR8 strain (MOI=0.1) at a concentration of 100 mM , 12h later, Trizol lysed cells, extracted RNA, reverse transcribed into cDNA, and detected influenza virus RNA copy number by Real-Time PCR ( figure 1 A). figure 1 B, with figure 1 A FAC and AIS were added to HELA cells in the same manner.

[0106] Results: Iron ions could inhibit the replication of influenza PR8 strain in A549 cells and HELA cells.

Embodiment 2

[0107] Example 2 Inhibitory effect of iron ions on influenza virus in mice

[0108] Methods: 6-8 week old C57BL / 6 female mice (60PFU / mice) were infected with influenza virus PR8 strain by intranasal instillation. While infected with the virus ( figure 2 A) or 30 minutes after infection ( figure 2 B) Nasal instillation of 30 μl AIS (5 mg / ml). Mice were weighed for 15 consecutive days after infection.

[0109] Results: After the administration of iron ions, the weight loss caused by virus infection could be significantly inhibited, indicating that iron ions have a therapeutic effect on influenza virus infection.

Embodiment 3

[0110] Example 3 Inhibitory effect of iron ions on Zika virus

[0111] Methods: Vero cells and U251 cells (MOI=0.1) were infected with Zika virus while adding FAC and FeCl 3 Or AIS (100 mM), Trizol lysed cells after 24h, 48h, 72h, extracted RNA, reverse transcribed into cDNA, and detected Zika virus RNA copy number by Real-Time PCR ( image 3 ).

[0112] Results: Different forms of iron ions were able to inhibit Zika virus replication.

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Abstract

The invention provides a use of iron ions, that is, an application in inhibiting RNA viruses. Specifically, the invention provides a use of iron ions, which is characterized in that it is used for preparing a pharmaceutical composition for inhibiting RNA viruses. The iron ion of the invention can effectively inhibit various RNA viruses, such as Flaviviridae virus, orthomyxovirus, etc., and the effect produced is the same as that of the compound preparation. Therefore, a variety of substances containing iron ions can be used to prevent RNA virus infection, so that the compound active ingredient of the present invention is very easy to prepare, simple and cheap, and has a strong application prospect.

Description

technical field [0001] The present invention relates to the field of biomedical treatment. Specifically, it relates to the application of iron ions alone in inhibiting RNA viruses, especially Orthomyxoviridae and Flaviviridae viruses. Background technique [0002] Influenza is an acute respiratory infection caused by the influenza virus. Influenza viruses belong to the Orthomyxoviridae family of RNA viruses, which are prone to mutation, are highly contagious and spread rapidly, and are very likely to cause widespread epidemics. Although there are antiviral drugs against influenza virus on the market, it is still necessary to develop new cheap and efficient antiviral drugs with different antiviral mechanisms. [0003] Zika virus (ZIKA virus) is a single-stranded positive-stranded RNA virus of the genus Flavivirus that is transmitted by mosquitoes. Typical symptoms of infection in adults include low-grade fever, maculopapular rash, joint pain, conjunctivitis, myalgia, heada...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/26A61P31/14A61P31/16
CPCA61K33/26Y02A50/30
Inventor 孟广勋王洪斌
Owner 中国科学院上海免疫与感染研究所
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