Bacillus velezensis as well as preparation method and application thereof

A technology of Bacillus velesi and Bacillus, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., to achieve the effect of easy operation, stable sensory quality, and prevention of material liquid deterioration

Active Publication Date: 2018-05-08
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus Velez Bacillus velezensis YGA1 is isolated from tobacco flavors and fragrances by using a screening medium. It has strong cellulase activity and a strong inhibitory effect on Escherichia coli. The application of this bacteria in feed liquid has not yet been published.

Method used

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  • Bacillus velezensis as well as preparation method and application thereof
  • Bacillus velezensis as well as preparation method and application thereof
  • Bacillus velezensis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation and Screening of Bacillus Velez YGA1

[0031] 1. Medium preparation: tryptic soy agar medium: tryptone 15 g / L, soytone 5 g / L, sodium chloride 5 g / L, agar 15 g / L, sterilized by high-pressure steam at 121°C for 15 min; Congo red cellulose medium: sodium nitrate 1 g / L, disodium hydrogen phosphate 1.2 g / L, potassium dihydrogen phosphate 0.9 g / L, magnesium sulfate 0.5 g / L, potassium chloride 0.5 g / L, yeast extract powder 0.5 g / L, acid hydrolyzed casein 0.5 g / L, Congo red 0.2 g / L, cellulose powder 5 g / L, agar 15.0 g / L, sterilized by high-pressure steam at 121°C for 15 minutes.

[0032] 2. Isolation and screening of strains

[0033] (1) Bacteria isolation: From several samples of flavor and fragrance, use tryptic soybean medium to separate bacteria by dilution coating plate method, pick a single colony and get a pure strain by streaking multiple times. Morphological judgment obtained 69 strains of pure bacteria.

[0034] (2) Screening of cellulase activity of stra...

Embodiment 2

[0042] Preparation of Concentrated Fermentation Broth of Bacillus Velez YGA1

[0043] 1. Induction and fermentation medium preparation: cellulose-containing tryptic soybean broth induction medium: tryptone 15 g / L, soytone 5 g / L, sodium chloride 5 g / L, cellulose powder 1-5 g / L, autoclaved at 121°C for 15 min;

[0044] 2. Obtaining of concentrated fermented liquid:

[0045] (1) Recovery and activation of YGA1 strain: take a glycerol tube to store the strain for a long time, recover it in a water bath at 37°C, or take a slant to store the strain for a short period of time, inoculate it in soybean broth supplemented with tryptone, at 30°C, 120-140rpm Cultivate for 20 hours to obtain seed culture solution;

[0046] (2) Expansion culture of YGA1 strain: inoculate the seed culture solution with 5% (v / v) inoculum in the tryptic soybean broth medium with 1g / L cellulose powder, cultivate at 30°C, 120-140rpm 24h, obtain the fermentation liquid of YGA1 bacterial strain;

[0047] (3) C...

Embodiment 3

[0049] Preparation of Concentrated Fermentation Broth of Bacillus Velez YGA1

[0050] 1 is the same as Example 2.

[0051] 2. Preparation of concentrated fermented liquid:

[0052] (1) Recovery and activation of YGA1 strain: take a glycerol tube to store the strain for a long time, recover it in a water bath at 37°C, or take a slant to store the strain for a short period of time, inoculate it in soybean broth supplemented with tryptone, at 30°C, 120-140rpm Cultivate for 18 hours to obtain seed culture solution;

[0053] (2) Expansion culture of YGA1 strain: inoculate the seed culture solution with 10% (v / v) inoculum in the tryptic soybean broth medium supplemented with cellulose powder 5g / L, cultivate at 30°C, 120-140rpm 32h, obtain the fermentation liquid of YGA1 bacterial strain;

[0054] (3) Collection and concentration of fermentation broth: Take 1L of YGA1 strain fermentation broth, centrifuge at 4°C, 12000rpm for 20-30min, collect the supernatant, and concentrate the ...

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Abstract

The invention relates to bacillus velezensis YGA1 as well as a preparation method and application thereof, and belongs to the technical field of microbe application. The bacillus velezensis YGA1 bacterial stain is preserved in CCTCC (China Center for Type Culture Collection) on November 17, 2017, and has the preservation code being CCTCC M 2017705. When the addition of the concentration fermentation liquid prepared from the YGA1 stain is 0.1 to 1.0 percent, the sensory quality of the material liquid can be effectively stabilized; the proliferation of decay bacteria in the material liquid is inhibited; the expiration date of the material liquid is prolonged; the cigarette sensory evaluation smoking effect shows that the addition quantity does not have influence on the cigarette sensory smoking quality. The method has the advantages that the cost is low; the implementation of the operation method is easy; great application potential is realized in aspects of prolonging the expiration date of the material liquid and preventing the material liquid deterioration.

Description

technical field [0001] The invention belongs to the technical field of microbial application, in particular to a kind of Bacillus veleisi Bacillus Velezensis YGA1, its preparation method and its application in prolonging the shelf life of feed liquid. Background technique [0002] Tobacco flavors and fragrances are widely used in tobacco and tobacco products, which can give cigarette products a unique style and are also an important factor in the style of cigarette brands. Feed liquid, also known as flavoring essence, is used in a large amount in the cigarette production process. It is generally composed of sugars such as sucrose, honey and fructose, natural extracts such as fruit concentrates, various additives such as glycerin and citric acid, and a large amount of water. . The complex composition and processing technology of the feed liquid lead to poor quality stability, easy deterioration, and a very short shelf life. In the actual production process of cigarette ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C11B9/00C12R1/07
CPCC11B9/00C12N1/02C12N1/20C12N1/205C12R2001/07
Inventor 夭建华米其利李晶朱昆楼牧梦张崇阳李雪梅陈建华杨光宇莫明和
Owner CHINA TOBACCO YUNNAN IND
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