A kind of extraction method of pineapple pomace polysaccharide

A technology of pineapple peel residue and extraction method, which is applied in the direction of anti-toxic agent and drug combination, can solve the problems of large investment in equipment and high operation requirements, and achieve the effects of less loss of nutrients, high yield of polysaccharides, and strong antioxidant activity

Active Publication Date: 2019-02-01
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with hot water extraction, ultrasonic-assisted extraction technology has the advantages of fast, efficient, time-saving and energy-saving, but it has high requirements for operation and large investment in equipment.

Method used

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  • A kind of extraction method of pineapple pomace polysaccharide
  • A kind of extraction method of pineapple pomace polysaccharide
  • A kind of extraction method of pineapple pomace polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Take 5g dry powder of pineapple pomace, add 150mL distilled water, 1.5% compound enzyme (xylanase: pectinase Y-23 mass ratio = 1:2), keep the natural pH of the extract (6.0), and spin Carry out the hydrolysis reaction in an evaporator, control the enzymolysis temperature at 50°C, the enzymolysis time for 2 hours, and the rotation speed at 130rpm / min. After the extraction, the enzyme is quenched in a boiling water bath for 10 minutes, centrifuged at 8000rpm for 10 minutes, the supernatant is taken, and the residue is added to 150mL of distilled water. Transfer it to a water bath for leaching at 100°C for 2 hours, centrifuge at 5000 rpm for 10 minutes after the extraction, take the supernatant, and repeat the extraction of the residue once. Concentrate the volume of the extract to 1 / 5 of the original volume by evaporation under reduced pressure, add 4 times the volume of 95% ethanol to the concentrate, let it stand overnight at 4°C, centrifuge at a speed of 5000r...

Embodiment 2

[0069] Example 2 Take 5g of pineapple pomace dry powder, add 150mL of distilled water, 2.0% compound enzyme (xylanase: pectinase Y-23 mass ratio = 1:2), keep the natural pH of the extract (6.0), and spin Carry out the hydrolysis reaction in the evaporator, control the enzymolysis temperature to 50°C, enzymolysis time to 1.5h, and the rotation speed to 130rpm / min. After the extraction is completed, the enzyme is inactivated in a boiling water bath for 10min, centrifuged at 6000rpm for 10min, the supernatant is taken, and the residue is added to 200mL of distilled water. Transfer it to a water bath for leaching at 100°C for 2 hours, centrifuge at 5000 rpm for 10 minutes after the extraction, take the supernatant, and repeat the extraction of the residue once. Concentrate the volume of the extract to 1 / 5 of the original volume by evaporation under reduced pressure, add 4 times the volume of 95% ethanol to the concentrate, let it stand overnight at 4°C, centrifuge at 4500r / min for ...

Embodiment 3

[0075] Embodiment 3 adopts the following steps to realize the present invention:

[0076] (1) Pretreatment: Freeze-dry the screened pineapple pomace, powder it, and pass through a 40-mesh sieve to obtain pineapple pomace dry powder; freeze-drying conditions: temperature -40°C, pressure 6Pa, time 24h;

[0077] (2) Degreasing: Put the dry powder of pineapple skin dregs obtained in step (1) into a container, add petroleum ether with a boiling range specification of 30-60°C to reflux and extract for 3 times, collect the filter residue, and dry it naturally for later use;

[0078] (3) Enzymatic hydrolysis of raw materials: add distilled water to the pineapple pomace powder prepared in step (2) at a ratio of 1:40g / mL to liquid, and at the same time add 2.2% compound enzyme (xylanase: pectinase Y- 23 mass ratio = 1:3), at 53°C, pH 6.0 in a water bath, carry out enzymatic hydrolysis in a rotary evaporator with a rotational speed of 130r / min for 2.1h, after enzymatic hydrolysis, boil t...

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Abstract

The invention discloses a method for extracting polysaccharide of pineapple pomace. The method for extracting the polysaccharide of the pineapple pomace comprises the following steps of pretreatment,degreasing, enzymolysis of a raw material, water extraction of filter residues, concentration, alcohol precipitation, deproteinization, depigmentation, drying and the like, wherein the extraction rateof the polysaccharide of the pineapple pomace can be improved effectively by adopting xylanase and pectinase Y-23 as a mixed enzyme preparation, then a trichloroacetic acid-butanol mixed solution isadded for deproteinization of crude polysaccharide, X-5 macroporous adsorption resin is added for the depigmentation of the deproteinized polysaccharide solution, and the purity of the polysaccharideof the pineapple pomace can be improved by controlling the conditions of the steps. The method for extracting the polysaccharide of the pineapple pomace is simple and rapid, and compared with a hot water extraction method, an ultrasonic wave-assisted extraction method and a cellulase extraction method, the obtained polysaccharide of the pineapple pomace has a high extraction rate, high purity andgood antioxidant activity, and can be used for preparing functional food, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of deep processing of horticultural crop products, and in particular relates to a method for extracting pineapple pomace polysaccharide. Background technique [0002] Pineapple (Ananas comosus), also known as pineapple and prosperous pear, is one of the four famous fruits in the tropics. According to incomplete statistics, the planting area of ​​pineapples in tropical regions of my country reaches 67,000 hm 2 , the output reached 1.44 million tons. During the fresh eating and processing of pineapple, a large amount of skin dregs will be produced, accounting for 15-20% of the fruit weight. The proportion of total sugar, water and citric acid is almost the same as that of the pulp. Due to the lack of processing technology and related research, a large amount of skin dregs are produced. Being discarded not only causes waste of resources, but also aggravates environmental pollution. [0003] Polysaccharide is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003
Inventor 胡会刚赵巧丽谢江辉胡玉林陈晶晶段雅婕
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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