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CK, CKMB, LDH and AST combined detection reagent

A combined detection and reagent technology, applied in the field of LDH and AST joint detection reagents, CK, CKMB, can solve the problems of inconvenient transportation and short storage time of liquid reagents

Active Publication Date: 2018-05-04
NINGBO MEIKANG BAOSHENG BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, most of the reagents used in the existing large-scale automatic biochemical analyzers are liquid reagents, and the liquid reagents have the disadvantages of short storage time and inconvenient transportation.

Method used

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  • CK, CKMB, LDH and AST combined detection reagent
  • CK, CKMB, LDH and AST combined detection reagent
  • CK, CKMB, LDH and AST combined detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The specific content of each component of the dilution solution 1 is:

[0039] Tris: 0.5mol / L, pH6.8

[0040] Triton X-100: 1.0%

[0041] Bilirubin oxidase: 10KU / L

[0042] Vitamin C oxidase: 20KU / L

[0043] Methylparaben: 2.0%

[0044] The concrete content of each composition of diluent 2 is:

[0045] Tris: 0.8mol / L, pH7.2

[0046] Inhibit CK-M antibody: 5.0g / L

[0047] The concrete content of each composition of freeze-dried pellet 1 is:

[0048] Tris: 0.4mol / L, pH7.5

[0049] α-KG: 2.0mol / L

[0050] NADH: 10mol / L

[0051] MDH: 20KU / L

[0052] L-Aspartic acid: 20mol / L

[0053] Bovine serum albumin: 10mol / L

[0054] The concrete content of each composition of freeze-dried pellet 2 is:

[0055] Tris: 0.5mol / L, pH8.5

[0056] L-lithium lactate: 10mol / L

[0057] NAD+: 20mol / L

[0058] Bovine serum albumin: 20mol / L

[0059] The concrete content of each composition of freeze-dried pellet 3,4 is:

[0060] Tris: 0.4mol / L, pH7.8

[0061] ADP: 10mol / L

[006...

Embodiment 2

[0071] The specific content of each component of the dilution solution 1 is:

[0072] Tris: 1.0mol / L, pH7.5

[0073] Brij-35: 5.0%

[0074] Potassium ferrocyanide: 10mol / L

[0075] Vitamin C oxidase: 50KU / L

[0076] Isobutylparaben: 5.0%

[0077] The concrete content of each composition of diluent 2 is:

[0078] Glycine-NaOH buffer: 1.0mol / L, PH7.8

[0079] Inhibit CK-M antibody: 10.0g / L

[0080] The concrete content of each composition of freeze-dried pellet 1 is:

[0081] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer: 1.0mol / L, pH7.5

[0082] α-KG: 5.0mol / L

[0083] NADH: 20mol / L

[0084] MDH: 50KU / L

[0085] L-aspartic acid: 40mol / L

[0086] Trehalose: 20mol / L

[0087] The concrete content of each composition of freeze-dried pellet 2 is:

[0088] N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer: 1.0mol / L, PH9.0

[0089] L-lithium lactate: 20mol / L

[0090] NAD+: 40mol / L

[0091] Trehalose: 40mol / L

[0092] The concrete content o...

Embodiment 3

[0113] The specific content of each component of the dilution solution 1 is:

[0114] Tris: 2.5mol / L, pH7.5

[0115] PEG: 5.0%

[0116] Potassium ferricyanide: 5mol / L

[0117] Vitamin C oxidase: 100KU / L

[0118]Isopropyl paraben: 10.0%

[0119] The concrete content of each composition of diluent 2 is:

[0120] 3-morpholine-2-hydroxypropanesulfonate sodium buffer: 0.5mol / L, pH8.0

[0121] Inhibit CK-M antibody: 15.0g / L

[0122] The concrete content of each composition of freeze-dried pellet 1 is:

[0123] 3-(Cyclohexylamine)-1-propanesulfonic acid buffer: 0.8mol / L, PH8.5

[0124] α-KG: 10mol / L

[0125] NADH: 100mol / L

[0126] MDH: 100KU / L

[0127] L-Aspartic acid: 100mol / L

[0128] Tween 80: 100mol / L

[0129] The concrete content of each composition of freeze-dried pellet 2 is:

[0130] N-(2-hydroxyethyl)piperazine-N'-4-butanesulfonic acid buffer: 1.0mol / L, PH10.0

[0131] L-lithium lactate: 50mol / L

[0132] NAD+: 100mol / L

[0133] Tween 80: 100mol / L

[0134] T...

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Abstract

The invention discloses a CK, CKMB, LDH and AST combined detection reagent which comprises a diluent 1, a diluent 2 and 4 freeze-dried balls. The diluent 1 comprises trismetyl aminomethane, a surfactant, a dehydrobilirubin interference agent, vitamin C oxidase and a preservative; the diluent 2 comprises a buffer solution ad an inhibitory CK-M antibody; the freeze-dried ball 1 comprises a buffer solution, alpha-ketoglutaric acid, a reduced coenzyme I, malic dehydrogenase, L-aspartate and a freeze-drying protective additive; the freeze-dried ball 2 comprises a buffer solution, L-lithium lactate,an oxidized coenzyme I and a freeze-drying protective additive; the freeze-dried balls 3 and 4 comprise a buffer solution, adenosine diphosphate, glucose-6-phosphate dehydrogenase, hexokinase, D-glucose, phosphocreatine, an enzyme activator and a freeze-drying protective additive. The combined detection reagent is applicable to a multifunctional full-spectrum POCT (Point-of-care Testing) biochemical analyzer, has good correlation to detection results of clinically common liquid reagents on a large biochemical analyzer, and has the advantages of being simple and convenient to operate, convenient in reagent preservation and transport, low in detection cost and the like.

Description

technical field [0001] The present invention relates to the technical field of in vitro diagnosis, in particular to a kind of CK (creatine phosphokinase or creatine kinase), CKMB (creatine kinase isoenzyme or CH-MB or serum creatine kinase isoenzyme), LDH (lactic acid dehydrogenase) and AST (aspartate aminotransferase) combined detection reagent. Background technique [0002] In the traditional myocardial enzyme spectrum, CK and CKMB appear early (3-8h) at the onset of acute myocardial infarction (AMI), have a short peak time (10-36h), and recover quickly (72-80h). LDH and AST appear late (8-18h) in the onset of AMI, take a long time to reach the peak (24-72h), and take a long time to recover (6-10d). CK and CKMB can be detected in the patient's blood in the early stage of the disease, before the myocardium has suffered extensive necrosis, and provide reliable information for the clinic at the first time. LDH and AST are of considerable value in observing the course of AMI...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/52C12Q1/50C12Q1/48C12Q1/32C12Q1/26G01N33/573
CPCG01N33/573C12Q1/26C12Q1/32C12Q1/485C12Q1/50C12Q1/52G01N2333/91188G01N2333/9123G01N2333/904
Inventor 张桂春李峰屏
Owner NINGBO MEIKANG BAOSHENG BIOMEDICAL ENG
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