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Bacterium cell breaking enzyme liquid sand wind prevention and control liquid and preparation method thereof

A bacterial cell-cracking enzyme and cell-cracking technology, applied in chemical instruments and methods, other chemical processes, organic fertilizers, etc., can solve the problems of poor desertification effect, large-scale treatment cost, and high processing cost, and achieve good durability , increase the production rate, improve the effect of enzyme activity

Inactive Publication Date: 2018-05-04
HOHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still has the following defects: 1. The treatment cost is relatively high, and the cost of large-scale treatment is relatively high; 2. The effect of microbial treatment on sand particles is better, but the effect on desertified land mixed with clay particles is poor; 3. The use of microorganisms may cause concerns among ordinary people, which is not conducive to the promotion of the project

Method used

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Embodiment Construction

[0018] The present invention will be described in further detail below in conjunction with the examples.

[0019] A kind of bacterial cell-cracking enzyme liquid wind-sand prevention and control liquid of the present invention is characterized in that: it comprises the bacterial cell-cracking enzyme liquid and the inorganic cementing fluid treatment liquid with the volume ratio of 1:3-1:5; the bacterial cell-cracking enzyme liquid It is obtained by ultrasonically crushing the aerobic bacteria solution enriched and cultivated by adding buffer protection solution. The medium formula used in the enriched culture process is: yeast extract powder 15-25g / L, ammonium chloride 8-12g / L, six Nickel chloride hydrate 20-25mg / L, manganese sulfate monohydrate 10-15mg / L, the adjusted ph is 8.5-9; the inorganic cement liquid treatment liquid includes the following components: the concentration ratio is 2:1-1:2 Urea 0.2-2mol / L, calcium salt 0.2-2mol / L. The calcium salt is a soluble calcium sa...

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Abstract

The invention discloses a bacterium cell breaking enzyme liquid sand wind prevention and control liquid and a preparation method thereof. The preparation method comprises the following steps: carryingout enrichment culture on microorganism strains, then centrifuging bacterial liquor after the enrichment culture, taking precipitates, adding buffer protection fluid, shaking up, then carrying out cell breaking on the obtained mixed liquor, carrying out centrifugal treatment after the cell breaking, taking supernatant liquor, and then obtaining the bacterium cell breaking enzyme liquid; and uniformly mixing the bacterium cell breaking enzyme liquid with an inorganic consolidating liquid, so that the bacterium cell breaking enzyme liquid sand wind prevention and control liquid is obtained. Thebacterium cell breaking enzyme liquid sand wind prevention and control liquid disclosed by the invention contains some nutrient sources required by plant growth, is beneficial to coverage of vegetation after sand wind treatment and is good in durability. In a cell breaking process of the preparation method disclosed by the invention, other impurities and concomitants can not be produced, enzymatic activity per unit volume is improved, time cost is effectively reduced, bacterium culture material cost is saved, preparation process is simple and easy, and the preparation method is applicable toactual application.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and microbial solidification, and relates to a bacterial cell-cleaving enzyme liquid wind and sand prevention liquid and a preparation method thereof. Background technique [0002] With the development of human society, the ensuing environmental problems are becoming more and more serious. Among them, land desertification and desertification issues are being valued by more and more countries and regions. As one of the countries with the most serious desertification problems in the world, China's related governance work without delay. The first and most important step in combating desertification is to fix the flowing sand and prevent sandstorms. [0003] Existing windbreak and sand fixation technologies can be roughly divided into three categories, including: chemical sand fixation, physical sand fixation, and microbial methods. Chinese patent application (application number: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C09K105/00
Inventor 高玉峰何稼顾张翔杨恩杰汤昕怡
Owner HOHAI UNIV
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