Preparation method of novel sepsis animal model

Abstract

The invention discloses a preparation method of a novel sepsis animal model. On the basis of implementing experiments on SPF-level Balb / c mice which are 8 weeks and 25-30g in weight and which undergoadaptive breeding for 1 week, the method is characterized by comprising the following steps: 1, in an adaptive breeding and molding stage, enabling the mice to freely take food and water; in an environment that light / dark alternate illuminating is implemented by an interval of 12h and temperature and humidity are proper, marking 60 Balb / c mice conforming to a standard, half male and half female, wherein each mouse has a corresponding fixed and unique serial number; with the adoption of a random number table method, equally dividing the mice into two major groups, namely a female group and a male group, and further dividing each major groups into four sub-groups, namely A (n=6), B (n=8), C (n=8) and D (n=8). The obtained novel model is intervened by such drugs as cyclosporin A and the like,resulting in a low immune function of the animals (the mice), which conforms to a circumstance that people, who are low in immune function due to such reasons as surgeries, age and the like, suffer from sepsis, so that a natural occurrence and development process of the sepsis can be simulated better.

Description

technical field [0001] The invention relates to the field of animal medical models, in particular to a preparation method of a novel sepsis animal model. Background technique [0002] Sepsis refers to the systemic inflammatory response syndrome caused by infection, the incidence rate remains high, and patients with this disease are very prone to complicated with multiple organ dysfunction. The pathogenesis of sepsis is complex, involving a series of problems such as inflammatory response, endothelial injury, and coagulation disorders. It is the number one killer of patients in intensive care units. Hundreds of thousands of people in Europe and the United States die of sepsis every year. Establishing a standardized animal model of sepsis is necessary to explore the pathophysiological mechanisms and preventive measures of severe systemic infection, septic shock and multiple organ dysfunction syndrome. [0003] Researchers have established a variety of sepsis animal models, wh...

AI Technical Summary

Problems solved by technology

In the past, animal models of sepsis were established, and the animals were all young and healthy, which deviated from clinical practice, which made it difficult for the results of animal model research to be applied in clinical sepsis treatment

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