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Multivalent meningococcus conjugated vaccine and preparation method thereof

A meningococcal and conjugation technology, applied in the biological field, can solve the problem of low expression level of fHbp protein, achieve the best immune effect and reduce the risk

Active Publication Date: 2018-04-27
BRAVOVAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] However, the use of fHbp protein alone also has certain defects, because the expression level of fHbp protein is low in some MenB strains, and a vaccine with only fHbp antigens cannot be used for a wide range of immune meningococcal infections. Fusion expression of multiple antigens may well be a good choice

Method used

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  • Multivalent meningococcus conjugated vaccine and preparation method thereof
  • Multivalent meningococcus conjugated vaccine and preparation method thereof
  • Multivalent meningococcus conjugated vaccine and preparation method thereof

Examples

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Embodiment Construction

[0046] A multivalent meningococcal conjugate vaccine based on the ΔfHbp-NadA protein carrier provided by the present invention will be further described below in conjunction with specific examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0047] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0048] 1. Cloning and prokaryotic expression of ΔfHbp and ΔfHbp-NadA recombinant proteins

[0049] 1. Construction of Pet-ΔfHbp and Pet-ΔfHbp-NadA recombinant plasmids

[0050] fHbp is a membrane surface lipoprotein, and many strains of meningococcus have its gene, and strains that do not carry the gene have also been found. According to the antigenic cross-reactivity and sequence similarity of the whole...

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Abstract

The invention provides recombinant delta fHbp-NadA fusion protein carrier-based multivalent meningococcocci. Through a flexible linker peptide chain, two active proteins are fused and expressed, activity of the two active proteins is kept and the active proteins are integrated into a single protein so that industrial production and immunization are controllable, a risk is greatly reduced and morepolysaccharide-protein binding sites are provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a multivalent meningococcal conjugate vaccine and a preparation method thereof. Background technique [0002] 1. Pathogenic microorganisms and vaccines [0003] Microorganisms that can cause infectious diseases in humans or animals are called pathogenic microorganisms or pathogenic microorganisms. Infection refers to the process of pathogenic microorganisms invading the body, growing and multiplying in certain parts, and causing a series of pathophysiological processes. When pathogenic microorganisms invade the body, the pathogenic microorganisms interact with the body and change each other's activity and function. Therefore, whether they can cause infectious diseases depends on the pathogenicity or virulence of the pathogenic microorganisms on the one hand, and on the other hand. Depends on the body's resistance, that is, immunity. The ability of pathogenic bacteria to cause infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/095A61P31/04C12N15/70C12N15/62C07K19/00
CPCA61K39/09A61K39/095A61K39/116A61K39/385A61K2039/57A61K2039/6031A61K2039/6068A61K2039/62A61K2039/70C07K14/22C07K2319/00C12N15/62C12N15/70A61K2300/00
Inventor 史晋李津吴克王文灏
Owner BRAVOVAX
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