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Immunohistochemical detection kit used for prognosis after hepatectomy

A detection kit and immunohistochemical technology, applied in the biological field, can solve the problems of ignoring the prognosis of myeloid cells and the important impact of anti-tumor treatment, failing to fully reflect the actual situation, and poor accuracy, so as to achieve mature test methods and ensure The effect of accuracy and simplicity of the detection process

Active Publication Date: 2018-04-20
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology has the following deficiencies: (1) the above-mentioned traditional clinicopathological diagnosis or single molecular markers are less effective and less accurate in judging the prognosis of patients after surgery; (2) the current methods for indicating the immune response in liver cancer Most of the molecular index models use lymphocyte markers, while ignoring the important impact of myeloid cells on patient prognosis and anti-tumor treatment; (3) Although a single myeloid cell marker can also predict patient prognosis to a certain extent, it cannot actually be comprehensive Reflect the actual situation of the myeloid cell response and even the overall immune response in the tumor

Method used

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  • Immunohistochemical detection kit used for prognosis after hepatectomy
  • Immunohistochemical detection kit used for prognosis after hepatectomy
  • Immunohistochemical detection kit used for prognosis after hepatectomy

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: Screening of antibodies used in the immunohistochemical detection kit of the present invention

[0020] The present invention selects three protein molecular markers of myeloid immune cells (CD169, CD33, CD11b), and these three markers have independent predictive ability for patient prognosis. Among them, CD169 is used to mark CD169 + of myeloid cells, CD33 was used to label CD33 + myeloid cells, CD11b is used to mark CD11b + of myeloid cells.

[0021] Table 1. Sources of antibodies and specific experimental conditions

[0022] Mark

Antibody source

Repair method

Color development time

CD33

Leica, Cat. No.: NCL-L-CD33

High pressure 10min, EDTA (pH9.0)

10min

CD11b

Abcam, Item No.: ab52478

High pressure for 10min, 10mMCB (pH6.0)

0.5min

CD169

R&D, Item No.: AF5197

Microwave 20min, EDTA (pH9.5)

5min

[0023] EDTA: ethylenediaminetetraacetic acid; CB: citrate buffer.

[0024] Th...

Embodiment 2

[0030] Embodiment 2: The double-antibody and triple-antibody combination scheme of the immunohistochemical detection kit of the present invention

[0031] The preparation method of the immunohistochemical detection kit of the present invention is the same as that of conventional immunohistochemical detection kits, except that the protein molecules detected by the first antibody used in the kit are: CD33, CD11b, and CD169. These three primary antibodies can detect clear positive signals in paraffin sections of liver cancer tissues.

[0032] For the immunohistochemical detection kit described in the present invention, its effectiveness is tested through the following steps.

[0033] (1) Specimen testing

[0034] 1. Select 465 cases of liver cancer tissue wax blocks containing tumor areas, and ensure that there is no large area of ​​necrosis;

[0035] 2. After selecting points for each sample of wax blocks, arrange them into a chip array, obtain 4 μm tissue chip paraffin sectio...

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Abstract

The invention relates to an immunohistochemical detection kit used for prognosis after hepatectomy. The immunohistochemical detection kit comprises a first antibody and a second antibody, the first antibody is two or combination of more than two selected from the following protein molecular markers of an antibody: CD33 (cluster of differentiation 33), CD11b (ITGAM), and CD169 (sialic acid adhesin). The kit employs a double antibody / tri-antibody combination index, prognosis condition of the patient after operation can be accurately predicted, so that appropriate treatment population and modes can be selected for doctors, and the kit provides more powerful support for effective diagnosis and treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an immunohistochemical detection kit for prognosis after resection of liver cancer, in particular to an immunohistochemical combination immunohistochemistry for predicting the overall survival of patients with hepatocellular carcinoma after resection. Histochemical detection kit. Background technique [0002] Primary hepatocellular carcinoma (liver cancer for short) is a common malignant tumor with the sixth and third highest morbidity and mortality rates, and China is a high-incidence area for liver cancer. In recent years, the incidence of liver cancer has a rising trend, seriously endangering human health and life safety. HCC is prone to vascular invasion and metastasis, which is the main reason for its poor prognosis and short survival period. Therefore, effectively predicting the overall survival of patients after surgery will help to select the appropriate treatment population ...

Claims

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 郑利民陈敏山吴翀林洁张耀军周仲国
Owner SUN YAT SEN UNIV CANCER CENT
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