Zygosaccharomyces bailii used for control of postharvest diseases of fruits and vegetables as well as preparation and application method thereof

A Bayer Zygomyces and postharvest disease technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, and preservation of fruits and vegetables, can solve the problem that the biocontrol effect is only verified on a small number of fruits, and there is a lack of antibacterial spectrum strains, etc. problems, to achieve significant social and ecological benefits, stable properties, and a wide antibacterial spectrum

Active Publication Date: 2018-04-20
南京万拓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Zygosaccharomyces bailii used for control of postharvest diseases of fruits and vegetables as well as preparation and application method thereof
  • Zygosaccharomyces bailii used for control of postharvest diseases of fruits and vegetables as well as preparation and application method thereof
  • Zygosaccharomyces bailii used for control of postharvest diseases of fruits and vegetables as well as preparation and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Biological characteristics of Zygosaccharomyces bailii strain BY39

[0019] 1. Morphological characteristics

[0020] (1) YPDA medium (yeast extract 1%, peptone 2%, glucose 2%, agar 1.8%, 121°C sterilization for 20 minutes) cultured at 26°C for 48h, the colonies were round and white, and the edges of the colonies were smooth and round. The cell shape is ellipsoid.

[0021] (2) After 24 hours of culturing in YPDA liquid medium, there is no formation of glutinous bacteria, the bacterial liquid is turbid, and there are sediments. Microscopically, the yeast cells are oval and budding.

[0022] 2. Molecular biology identification

[0023] Use universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to PCR amplify the nucleic acid sequence of yeast 26S rDNA D1 / D2 region, and the PCR product The sequencing results are entered into the website www.NCBI.nlm.nih.gov for BLAST, homologous sequences are downloaded from th...

Embodiment 2

[0025] Example 2 The inhibitory effect of Bayer joint yeast BY39 on apple black spot

[0026] 1. Experimental protocol

[0027] Take out the Bayer joint yeast BY39 from the refrigerator at -80°C, activate it with YPDA medium (10g yeast extract, 20g peptone, 20g glucose, 18g agar, 1000ml deionized water, natural pH, sterilized at 121°C for 30min) and activate it. The colonies were placed in YPD liquid medium, cultured at 26°C, 200r / min for 24h, centrifuged at 4000rpm for 5min, discarded the supernatant, the collected bacteria were washed 3 times with sterile water, and counted on a hemocytometer to prepare the concentration 1×10 8 Cells / mL Bayer conjugative yeast BY39 bacterial suspension.

[0028] Activate Alternaria tenuissima on a PDA medium plate, culture it at 26℃ for 7 to 14 days, scrape appropriate amount of spores, and prepare the concentration with sterile water to 5×10 4 Cell / mL Alternaria spore suspension.

[0029] The healthy and undamaged apple fruit was sterilized with 2...

Embodiment 3

[0032] Example 3 The inhibitory effect of Bayer's joint yeast BY39 on grape disease after harvest

[0033] 1. Experimental protocol

[0034] Take out the Bayer joint yeast BY39 from the refrigerator at -80°C, activate it with YPDA medium (10g yeast extract, 20g peptone, 20g glucose, 18g agar, 1000ml deionized water, natural pH, sterilized at 121°C for 30min) and activate it. The colonies were placed in YPD liquid medium, cultured at 26°C, 200r / min for 24h, centrifuged at 4000rpm for 5min, discarded the supernatant, the collected bacteria were washed 3 times with sterile water, and counted on a hemocytometer to prepare the concentration 1×10 8 Cells / mL Bayer conjugative yeast BY39 bacterial suspension.

[0035] Activate Botrytis porri, Aspergillus aculeatus or Fusarium redolens on a PDA medium plate, culture for 7 to 14 days at 26℃, scrape appropriate amount of spores, and prepare with sterile water The concentration is 5×10 4 Cells / mL of a spore suspension of Botrytis cinerea, Aspe...

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PUM

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Abstract

The invention discloses a Zygosaccharomyces bailii bacterial strain BY39 used for control of postharvest diseases of fruits and vegetables and with broad antibacterial spectrum and stable effect and an application method thereof. The bacterial strain is preserved in CGMCC with the preservation number of CGMCC No.14912. The application method of the grape wine saccharomyces cerevisiae comprises thefollowing steps: activating the bacterial strain, carrying out fermental cultivation with YPD, centrifuging, and preparing bacterium suspension with the concentration of 1*10<8> cells / mL from thalliwith sterile water; putting fruits and vegetables such as apples, grapes, strawberries, citrus and cherry tomatoes into the bacterium suspension, soaking for 30 seconds, then taking out, and carryingout air drying; and putting into a preservation box, and storing at room temperature. The Zygosaccharomyces bailii bacterial strain disclosed by the invention can control black spot of the apples, botrytis, aspergillosis and fusariosis of the grapes, botrytis and aspergillosis of the strawberries, penicilliosis of the citrus as well as botrytis and aspergillosis of the cherry tomatoes at the sametime, and loss caused by the postharvest diseases is reduced, so that the Zygosaccharomyces bailii bacterial strain disclosed by the invention has a good application prospect.

Description

Technical field [0001] The present invention relates to the field of biological control of postharvest diseases of fruits and vegetables, and in particular to a Zygosaccharomyces bailii strain for biological control of postharvest diseases of fruits and vegetables. This strain is the main postharvest strain for apples, grapes, strawberries, citrus, and cherry tomatoes. The diseases have significant control effects. Background technique [0002] Although the quality of fresh fruits and vegetables is affected by many factors, disease is the main cause. Among them, the rot caused by fungal diseases is the most serious factor in the loss of fruit after harvest. Although many methods such as agricultural control, physical control, chemical control and biological control can be used to prevent and control postharvest diseases of fruits and vegetables, the current main measure is chemical control (Eckert & Ogawa, 1985, 1988). However, long-term use of chemical pesticides not only lead...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155C12N1/16C12N1/145C12R2001/645
Inventor 王友升王颖马琳黄津津范雅为姚婷
Owner 南京万拓生物科技有限公司
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