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Synbiotic preparation

A technology of synbiotics and preparations, applied in the direction of bacteria used in food preparation, food shaping, food science, etc., can solve the problems of inability to protect probiotics from gastric acid digestion and limited protection of probiotic activity, etc. Achieve the effect of protecting the activity of probiotics, promoting the growth of probiotics, maintaining normal peristalsis and defecation

Inactive Publication Date: 2018-04-13
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Common synbiotic preparations on the market are usually delivered in a mixed form of liquid prebiotics and probiotics, which cannot well protect probiotics from gastric acid digestion; some synbiotics are prepared by reducing water activity, The products produced in this way are mostly in the form of powder, which has a very limited protective effect on the activity of probiotics in the digestive tract.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare carbon source-free MRS liquid medium (peptone 20g / L, beef extract 20g / L, yeast extract 10g / L, sodium acetate 6.03g / L, Tween 80 2g / L, triammonium citrate 4g / L, sulfuric acid Magnesium (MgSO 4 ·7H2O) 0.8g / L, manganese sulfate (MnSO4·H2O) 0.23g / L, dipotassium hydrogen phosphate 4g / L, pH6.2), take probiotic capsules (each containing Lactobacillus rhamnosus 1.1x10 11 CFU, Lactobacillus paracasei 4.5x10 10 CFU, Lactobacillus plantarum 4.2x10 10 CFU, Bifidobacterium (Bl04) 5.1x10 10 CFU, Lactobacillus salivarius 1.7x10 10 CFU, Streptococcus thermophilus 1.6x10 10 CFU, Bifidobacterium animalis 1.0x10 10 CFU, Bifidobacterium longum 1.3x10 10 CFU, Bifidobacterium breve 8.5x10 9 CFU, Bifidobacterium infantis 3x10 9 CFU, Lactobacillus germanus 3x10 9 CFU, Lactobacillus reuteri 3x10 9 CFU) 1 grain, take the bacterial powder and disperse it into 50mL of the above medium, and inoculate 100uL per well in a 96-well plate. The above-mentioned inoculated wells were divi...

Embodiment 2

[0028] Take 1000g of dried brown algae, break it into algal mud after rehydration, add 50 times the volume of deionized water, use 1% cellulase to hydrolyze at 50°C for 30 minutes, heat at 98°C for 3h to inactivate the enzyme, filter the residue to collect the solution, add 1 / 4 volume of ethanol, the precipitate was collected and dried. Weigh the above-mentioned dried precipitate to prepare an aqueous solution with a mass fraction of 5%, stir to make it swell, then add concentrated hydrochloric acid to a final concentration of 0.3mol / L, place it in an oven at 85°C for 5 hours, discard the supernatant, and pour Add 1.5% sodium carbonate aqueous solution to the precipitation to neutrality, 50 ℃ water bath for 1 hour, adjust the pH to 2.8, collect the precipitate, add 1.5% sodium carbonate aqueous solution again, add ethanol to the obtained supernatant after adjusting neutrality The final concentration was 90%, and the collected precipitate was dialyzed and freeze-dried to obtain...

Embodiment 3

[0032] Weigh food-grade sodium alginate to prepare an aqueous solution with a mass fraction of 5%, stir to make it swell, then add concentrated hydrochloric acid to a final concentration of 0.3mol / L, place it in an oven at 90°C for 3 hours, discard the supernatant, and pour Precipitation Add 1.5% sodium carbonate aqueous solution to neutrality, 50 ° C water bath for 2 hours, adjust pH to 2.8, collect the precipitate, add 1.5% sodium carbonate aqueous solution again, add ethanol precipitation after the obtained supernatant is neutralized , collect the precipitate and freeze-dry to obtain polyguluronic acid after dialysis. Polyguluronic acid deionized water prepared 12% concentration solution, adding xylooligosaccharide, food grade calcium carbonate and probiotic powder to the final concentration of 2%, 2% and 10% respectively 10 cfu / mL. Take vegetable oil, add 1 / 5 volume of glacial acetic acid, add 1.25% Tween 80, stir evenly and add the above-mentioned polyguluronic acid solu...

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Abstract

The invention provides a synbiotic preparation. The synbiotic preparation is a microsphere preparation prepared from polyguluronic acid and probiotics. The synbiotic preparation adopts the polyguluronic acid as a carrier material, the polyguluronic acid has an anti-digestion characteristic and can promote growth of the probiotics, and as a carrier of active probiotics, the polyguluronic acid can protect the probiotics from digestion of gastric acid and digestive enzyme in the alimentary tracts, so the viable count of the probiotics arriving the intestinal tracts is increased; and an embeddingmaterial used can promote growth of the probiotics and has double effects of protecting the activity of the probiotics and promoting proliferation of the probiotics in the intestinal tracts.

Description

technical field [0001] The invention relates to a synbiotic and its preparation, belonging to the field of food and biotechnology. Background technique [0002] Human health is closely related to the structure of the intestinal flora. Gut microbes influence body weight and digestion, protect against infection and reduce the risk of autoimmune diseases, and control the body's response to cancer treatment drugs. Bifidobacterium, Lactobacillus and other probiotics can synthesize a variety of vitamins necessary for human growth and development, such as B vitamins, vitamin K, niacin, pantothenic acid, etc.; they can also use protein residues to synthesize essential amino acids, such as aspartic acid, Phenylalanine, valine and threonine, etc.; participate in the metabolism of carbohydrates and proteins; synthesize short-chain fatty acids; also promote the absorption of iron, magnesium, zinc and other mineral elements; inhibit the growth of pathogenic bacteria , plays an importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125A23L33/135A23P10/20A23P10/30
CPCA23L33/125A23L33/135A23P10/20A23P10/30A23V2002/00A23V2400/165A23V2400/173A23V2400/125A23V2400/513A23V2400/515A23V2400/517A23V2400/519A23V2400/175A23V2400/529A23V2400/113A23V2400/169A23V2400/533A23V2200/3204A23V2250/51A23V2250/28
Inventor 宋悦凡汪秋宽辛泉伯崔仲元武龙何云海
Owner DALIAN OCEAN UNIV
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