Identification method of snake bile by ultra-high performance liquid chromatography-tandem mass spectrometry

Abstract

The invention discloses a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) identifying method of snake bile. By means of the HPLC-MS / MS method, a snake bile specificity identifying method is established. A sample is taken and directly diluted and then subjected to mass spectrometry, detection is conducted under a multiple reaction monitoring (MRM) mode, and whether snake bile is contained or not is judged according to feature components of the snake bile. The HPLC-MS / MS method is rapid and sensitive and can accurately identify whether the sample contains the snake bile or not, and a high-specificity and high-repeatability quality control method of the snake bile is established.

Description

technical field [0001] The present invention relates to Chinese patent medicine herbs The technical field of identification, specifically the identification method of snake bile by ultra-high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Snake bile has the effects of resolving phlegm and spasm, clearing heat and detoxification, clearing liver and improving eyesight. It is used for phlegm obsessed with heart orifices, wind-heat madness, cough with excessive phlegm, cloudy eyes, red and swollen hemorrhoids, heat toxin on the skin, and prickly heat. It is a commonly used Chinese patent medicine snake gallbladder The main raw material of Chuanbei liquid, Shelian Chuanbei powder, Niuhuang Shedan Chuanbei liquid (capsules, dripping pills), etc. Due to the unique medicinal value of snake bile, coupled with the large market demand, diminishing natural resources, and high price, the adulterated substances that have been reported include ch...

AI Technical Summary

Problems solved by technology

Due to the unique medicinal value of snake bile, coupled with the large market demand, diminishing natural resources, and high price, the adulterated substances that have been reported include chicken, duck, fish, pig, cattle and other animal bile, starch paste, and vegetable juice. , gelatin solution, pigment, and Rehmannia glutinosa extract, etc., as well as oil gallbladder (use oil to stimulate the snake's nasal cavity or snake extract, so that the bile increases and the quality is poor) or honey gallbladder, or soak the snake gallbladder for many times, and then separate the snake gallbladder from the wine liquid For sale makes bile thin and poor quality

The current local standards all use sodium taurocholate as the distinguishing feature to identify snake bile, but the content of sodium taurocholate in the bile of other animals such as cattle, sheep, chicken, dog and pig is relatively high, and the identification method is not specific

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