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A method for measuring the release of heavy metal elements in snus

A measurement method and technology of snus, which is applied in the field of chemical analysis and detection, can solve the problems of unscientific quality and safety evaluation of snus products, low release amount, etc.

Active Publication Date: 2020-04-14
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, snus will not be completely contained in the process of ingestion, and the heavy metal elements in it will only partially dissolve after contacting human saliva, and the actual release amount is far lower than its own background content, so only the total amount is used as an indicator. It is impossible to evaluate the quality and safety of snus products scientifically and objectively

Method used

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  • A method for measuring the release of heavy metal elements in snus
  • A method for measuring the release of heavy metal elements in snus
  • A method for measuring the release of heavy metal elements in snus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Measurement method

[0058] 1.1 Sample pretreatment

[0059] Accurately weigh the snus sample and place it in the solid-phase extraction empty column, accurate to 0.1mg, then put the solid-phase extraction column into the designated position of SPE, after cleaning the pipeline, slowly add artificial saliva to the sample as eluent Solid-phase extraction, collecting the liquid after washing. Wherein, the solid-liquid ratio of the added weight of the snus sample to the added volume of the artificial saliva is 2-12:20, g / ml.

[0060] Set the conditions of solid phase extraction, specifically: sample loading time: 15-25min; number of channels: 3-5 channels; pipeline cleaning solution: ultrapure water; volume of pipeline cleaning solution: 5-15mL; The extraction rate: 5-15mL / min; the cleaning rate of the pipeline cleaning solution: 5-15mL / min; the cleaning times of the pipeline cleaning solution: 2-4 cycles; the eluent: artificial saliva; the volume of the eluent : 1-3m...

Embodiment 2

[0073] 1. Measurement method

[0074] 1.1 Sample pretreatment

[0075] Accurately weigh the snus sample and place it in the solid-phase extraction empty column, accurate to 0.1mg, then put the solid-phase extraction column into the designated position of SPE, after cleaning the pipeline, slowly add artificial saliva to the sample as eluent Solid-phase extraction, collecting the liquid after washing. Wherein, the solid-liquid ratio of the added weight of the snus sample to the added volume of the artificial saliva is 4-10:20, g / ml.

[0076] Set the conditions of solid phase extraction, specifically: sample loading time: 20min; number of channels: 4 channels; pipeline cleaning solution: ultrapure water; volume of pipeline cleaning solution: 10mL; extraction rate of pipeline cleaning solution: 10mL / min; cleaning rate of pipeline cleaning solution: 10mL / min; cleaning times of pipeline cleaning solution: 2 cycles; eluent: artificial saliva; volume of eluent: 2mL; extraction rate...

Embodiment 3

[0092] 1. Standard working curve, detection limit and quantification limit

[0093] According to 1.2 in Example 2, a series of mixed element standard solutions of chromium, nickel, arsenic, selenium, cadmium and lead with different concentrations were prepared, and the specific concentration ranges are shown in Table 2. Simultaneously, prepare germanium by 1.2 in embodiment 2 72 ,indium 115 ,bismuth 209 Mixed element internal standard solution.

[0094] A series of standard solutions with different concentrations prepared above and the internal standard solution are respectively according to the provisions of 1.3 in Example 2, and ICP-MS detection is carried out synchronously, and the mass-to-charge ratio intensity ratio of the corresponding element / corresponding internal standard element is the ordinate (Y axis), the mass concentration ratio of its corresponding corresponding element / corresponding internal standard element is abscissa (X axis), carries out regression analy...

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Abstract

The invention provides a method for determining the release amount of heavy metal elements in an oral cigarette. The method comprises the following steps that 1, sample pretreatment is performed, wherein an oral cigarette sample is weighed, artificial saliva is adopted as leacheate, liquid obtained after elution is collected after solid-phase extraction, and transfer and volume fixation are performed to obtain a sample solution; 2, standard solutions are prepared, wherein germanium 72, indium 115 and bismuth 209 internal standard solutions and chromium, nickel, arsenic, selenium, cadmium and lead element standard solutions are prepared; 3, determination is performed, wherein inductively coupled plasma mass spectrometry detection is conducted on the sample solution, the standard solutions and the internal standard solutions, an internal standard synchronization standard curve method is adopted for quantification, and the content of chromium, nickel, arsenic, selenium, cadmium and lead in the sample solution is obtained. The method for determining the release amount of heavy metal elements in the oral cigarette simulates the smoking process of the oral cigarette, is good in association coefficient, recovery rate and repeatability and fills the blank on the domestic relevant detection technology aspect.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and relates to a method for measuring the release amount of heavy metal elements in snus. Background technique [0002] Chromium, nickel, arsenic, selenium, cadmium, and lead are common heavy metal elements that have received extensive research and attention in recent years due to their greater harm to humans and the environment. Chromium, nickel, arsenic, selenium, cadmium, and lead in tobacco are all harmful components in the Hoffmann list. Snus is a kind of smokeless tobacco product made by adding appropriate amount of spices, salt (sodium chloride), water and humectant to tobacco powder and processed by heat treatment. It is usually packed in non-woven bags, and the finished product is In small tea bag form. Snus is put directly into the human mouth when ingested, usually placed between the gums and cheeks, mainly in contact with human saliva and capillaries. cause ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/73
CPCG01N21/73
Inventor 陆怡峰张珲姿李永霞姚鹤鸣顾文博刘广超
Owner SHANGHAI TOBACCO GRP CO LTD
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