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Construction method for light-induced electrochemical immunosensor for detecting double tumour markers

An immunosensor and tumor marker technology, applied in the field of photoelectrochemical immunosensor construction, can solve the problems of fast recombination rate and poor photoelectric conversion efficiency, and achieve the effects of inhibiting recombination, improving sensitivity, promoting separation and hole transport

Inactive Publication Date: 2018-03-30
UNIV OF JINAN
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Problems solved by technology

But B-TiO 2 The recombination rate of photogenerated electron-hole pairs in NRs is too fast, resulting in poor photoelectric conversion efficiency

Method used

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Embodiment Construction

[0015] In order to further understand the present invention, carry out according to the technical scheme of the present invention in conjunction with embodiment, provide specific implementation mode:

[0016] (1) Preparation of titanium dioxide nanorods. Add 5-20 mL hydrochloric acid (12 M) into 5-20 mL ultrapure water, stir at room temperature for 10 min, add 0.2-10 mL tetra-n-butyl titanate solution, and continue stirring for 30 min to obtain a mixed solution; fluorine-doped SnO 2 The transparent conductive glass (FTO) was washed in acetone, ethanol, and ultrapure water for 10 minutes in sequence, and after drying, it was placed in the polytetrafluoroethylene lining of the autoclave with the conductive side facing down, and the mixed solution was transferred to the lining. , close and tighten the reactor, and react in a preheated oven at 150 °C for 30-300 min at a constant temperature; after the reaction is completed, cool the autoclave to room temperature naturally, take ou...

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Abstract

The invention discloses a construction method for a double-component light-induced electrochemical immunosensor based on a dendritic titanium dioxide nano-rod-strontium titanate (B-TiO2 NRs-SrTiO3) hetero-junction, and through the method, detecting alpha-fetoprotein and carbohydrate antigen 153 simultaneously an be achieved. The B-TiO2 NRs-SrTiO3 hetero-junction is synthesized by utilizing a hydrothermal method, so that carrier separation and hole transportation are promoted, and the photocurrent density produced by the B-TiO2 NRs-SrTiO3 is remarkably increased by compared with that produced by B-TiO2 NRs. On the basis of the B-TiO2 NRs-SrTiO3 hetero-junction, beta-galactosidase and acetylcholin esterase are taken as a signal marker for specifically catalyzing hydrolysis of p-aminobenzypylgalactoside and acetylcholine to produce p-aminophenol and choline in situ to serve as sacrificial electron donors respectively for distinguishing photocurrent signals. Because of proper signal difference, the sensor can realize high-sensitivity detection of the alpha-fetoprotein and the carbohydrate antigen 153.

Description

technical field [0001] The invention relates to the field of quantitative detection of dual tumor markers, in particular to the construction of a photoelectrochemical immunosensor for simultaneous and specific detection of two tumor markers based on dendritic titanium dioxide nanorod-strontium titanate heterojunction. The invention also relates to the field of synthesis of inorganic nanocomposites. Background technique [0002] Tumor markers are substances synthesized and secreted by tumor cells through gene expression, or abnormally produced by the body in response to tumors, and play an important role in the early diagnosis of tumors. Timely detection of the content of tumor markers is of great significance for clinical tumor screening and early diagnosis, so it is necessary to construct a highly sensitive immunosensor for the detection of tumor markers. Most of the reported immunosensors are used to detect single tumor markers, and only a few immunosensors are used to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/531G01N33/543G01N27/327C01G23/053C01G23/00B82Y15/00
CPCB82Y15/00C01G23/006C01G23/053C01P2004/16G01N27/3275G01N33/531G01N33/54386G01N33/57484
Inventor 于京华薛洁高超民葛慎光颜梅
Owner UNIV OF JINAN
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