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Serum marker for detecting pulmonary embolism and application thereof

A technology of serum markers and pulmonary embolism, applied in the field of medicine and biology, can solve problems such as unclear diagnosis of pulmonary embolism

Active Publication Date: 2018-03-27
BEIJING INST OF HEART LUNG & BLOOD VESSEL DISEASES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it is not clear how to use these inflammatory factors to quickly and accurately diagnose pulmonary embolism, and its predictive role in the occurrence of APE

Method used

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  • Serum marker for detecting pulmonary embolism and application thereof
  • Serum marker for detecting pulmonary embolism and application thereof
  • Serum marker for detecting pulmonary embolism and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Differential verification of serological markers

[0030] Randomly grouped according to gender and age, 32 healthy people were selected as controls, and 31 patients with aortic aneurysm / aortic dissection were selected. The expression levels of protein factors in the extracted serum samples were detected using the QAH-CUST chip.

[0031] Experimental steps:

[0032] 1. Complete drying of the slide chip: Take the slide chip out of the box, and after equilibrating at room temperature for 20-30 minutes, open the packaging bag, uncover the sealing strip, and then put the chip in a vacuum desiccator or dry at room temperature for 1- 2 hours.

[0033] 2. Configuration of standard products:

[0034] (1) Add 500 μL of sample diluent to the vial of cytokine standard mixture and redissolve the standard. Before opening the vial, give it a quick centrifuge and gently pipette up and down to dissolve the powder. Label this vial as Std 1.

[0035] (2) Mark six clean cent...

Embodiment 2

[0069] Example 2, large sample verification of pulmonary embolism serological markers

[0070] Randomly grouped according to gender and age, 101 healthy people were selected as controls, 234 patients with aortic aneurysm / aortic dissection, and 203 patients with pulmonary embolism. The ELISA experiment of serum protein markers was carried out, and the corresponding RayBiotechHuman ELISA Kit was used to detect the expression levels of the nine protein factors obtained in Example 1, to verify whether the histone factors could effectively distinguish patients with aortic aneurysm / aortic dissection.

[0071] The experimental steps are as follows:

[0072] 1. Reagent preparation

[0073] (1) Equilibrate the kit and samples to room temperature (18-25°C);

[0074] (2) Samples According to the pre-test results, the serum samples of the persons to be tested were diluted according to the multiples shown in Table 2 below.

[0075] Table 2. Dilution multiples when detecting different di...

Embodiment 3

[0110] Embodiment 3, optimal diagnosis combination protein factor analysis

[0111] Through the ROC statistical analysis of the combined effect of each factor, it is determined to distinguish aortic aneurysm / aortic dissection (A) from healthy people (H), pulmonary embolism (PE) from healthy people (H), aortic aneurysm / aortic dissection ( A) Best diagnostic combination with pulmonary embolism (PE).

[0112] Through further analysis, it is determined that the best diagnostic factor for distinguishing between pulmonary embolism (PE) and healthy people (H) is TRAIL, and the serum of 423 routine healthy people and the serum of 298 routine pulmonary embolism patients are selected again, according to embodiment 1-2 Methods Serological marker detection, the results are as follows in Table 6 or Figure 6 shown.

[0113] Table 6, the concentration of the serum marker TRAIL of healthy people and patients with aortic aneurysm / dissection

[0114]

Healthy person (H)

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Abstract

The invention relates to a serum marker for detecting pulmonary embolism and an application thereof. The serum marker can be used for distinguishing patients with pulmonary embolism and normal people,the serum marker is a protein factor, and the protein factor is TRAIL: a tumor necrosis factor-related apoptosis-inducing ligand.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, in particular, it relates to a serum marker for detecting pulmonary embolism and its application Background technique [0002] Acute pulmonary embolism (APE) is a relatively common and potentially life-threatening vascular disease clinically, and it is one of the three common fatal cardiovascular diseases. The diagnosis of APE currently mainly relies on imaging examinations, including pulmonary artery CT, pulmonary ventilation perfusion scan, and pulmonary angiography, etc., but the cost is high, especially pulmonary angiography is invasive. Therefore, its clinical application is limited. Therefore, biomarkers that have been widely used in clinical practice in recent years, such as D-dimer, brain natriuretic peptide, troponin, etc., have shown good application prospects. [0003] Currently, D-dimer is commonly used to assist in the diagnosis of APE. In acute thrombosis, coagulatio...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 杜杰李玉琳杨新颖刘卓惠
Owner BEIJING INST OF HEART LUNG & BLOOD VESSEL DISEASES
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