Preparation method of cell wax block on the basis of bloody cell specimen and the cell wax block

A cell wax block and sample technology, applied in the biological field, can solve the problems of affecting the quality of the wax block, affecting the diagnosis result, and long fixation time, etc., to achieve the effects of preventing interference, improving accuracy, and improving enrichment

Inactive Publication Date: 2018-03-23
ZHANG ZHOU HALTH VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the fixed time of the existing cell wax block is long, and the cell loss is obvious, which affects the diagnosis result. At the same time, due to the existence of a large number of red blood cells in the existing blood cell sample, the quality of the wax block and the diagnosis result are affected. For some difficult cases Immunohistochemical staining of cells will also affect the judgment of positive results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of cell wax block, it is made by following preparation method:

[0044]After the blood cell sample was washed with the first fixative solution, centrifuged for the first time at 3000r / min for 5min, and after standing for 10min, the precipitate and the supernatant 1cm away from the precipitate were collected, dispersed, and again at 3000r / min Centrifuge for 5 minutes under the condition of 3000r / min, discard the supernatant, collect the first precipitate sample, add the second fixative, mix evenly, and centrifuge for the second time under the condition of 3000r / min for 5min to obtain the second precipitate, dehydrate the second precipitate, and make it transparent , soaked in wax, embedded, and cut the embedded blood cell sample into thin slices with a thickness of 4um.

[0045] Wherein, the first fixative solution is ethanol acetic acid solution, and the ethanol acetic acid solution is obtained by mixing ethanol with a volume fraction of 25% and acetic acid at a ...

Embodiment 2

[0051] A kind of cell wax block, it is made by following preparation method:

[0052] Let the blood cell sample stand overnight, take the bottom 20ml blood cell sample and wash it with the first fixative, centrifuge for the first time at 3100r / min for 5min, collect the first sediment sample, add the second fixative, mix well Finally, centrifuge for the second time under the condition of 3100r / min for 5min to obtain the second precipitate. The second precipitate is dehydrated, transparent, soaked in wax, embedded, and the embedded blood cell sample is cut into thin slices with a thickness of 5um.

[0053] Wherein, the first fixative solution is ethanol acetic acid solution, and the ethanol acetic acid solution is obtained by mixing ethanol with a volume fraction of 25.5% and acetic acid at a volume ratio of 19:1.

[0054] The second fixative solution is a neutral buffered formaldehyde solution with a concentration of 3.5 wt% at a temperature of 4.5°C.

[0055] Dehydration incl...

Embodiment 3

[0059] A kind of cell wax block, it is made by following preparation method:

[0060] Let the blood cell sample stand overnight, take the bottom 30ml blood cell sample and wash it with the first fixative, centrifuge for the first time at 3100r / min for 6min, collect the first sediment sample, add the second fixative, mix well Finally, centrifuge for the second time under the condition of 2900r / min for 6min to obtain the second precipitate. The second precipitate is dehydrated, transparent, soaked in wax, embedded, and the embedded blood cell sample is cut into thin slices with a thickness of 3um.

[0061] Wherein, the first fixative solution is ethanol acetic acid solution, and the ethanol acetic acid solution is obtained by mixing ethanol with a volume fraction of 25.5% and acetic acid at a volume ratio of 18:1.

[0062] The second fixative solution is a neutral buffered formaldehyde solution with a concentration of 4 wt% at a temperature of 3.5°C.

[0063] Dehydration includ...

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PUM

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Abstract

The invention relates to a preparation method of a cell wax block on the basis of a bloody cell specimen and the cell wax block and relates to the technical field of biology. The preparation method includes the steps of: 1) washing the bloody cell specimen with a first immobilizing solution and performing initial centrifugation, collecting a first precipitate sample and adding a second immobilizing solution, uniformly mixing the liquid, and performing secondary centrifugation to prepare a second precipitate; 2) dehydrating the second precipitate, performing transparent treatment, soaking the second precipitate in wax and embedding the second precipitate. The first immobilizing solution is an ethanol-acetic acid solution prepared by mixing ethanol, volume fraction being 24-25.5%, and aceticacid according to the volume ratio of 18-19:1. The preparation method is high in preparation efficiency and has simple operation. The prepared cell wax block can be preserved permanently, contains large quantity of cell components, has complete cell structure, can form clear image under high-magnification background, and can effectively increase accuracy of diagnosis.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for preparing a blood cell sample-based cell wax block and the cell wax block. Background technique [0002] Exfoliative cytology examination is one of the effective methods for tumor diagnosis. The small amount of hydrothorax and ascites absorbed during the preparation of ordinary smears cannot represent the status of the entire hydrothorax and ascites. Examine most of the cells in the pleural and ascites in order to observe the morphology of most cells, and the cell wax block is easy to preserve, which greatly improves the sensitivity and specificity of cytopathological diagnosis, and makes up for the limited and insufficient production of conventional cell smears. The inadequacies of multiple stains warrant further research and prospective studies. [0003] However, the fixed time of the existing cell wax block is long, and the cell loss is obvious, which affects the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 唐忠辉方志达温路生
Owner ZHANG ZHOU HALTH VOCATIONAL COLLEGE
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