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Enzyme preparation that can be used for degumming of hemp and its bast degumming process of hemp

An enzyme preparation and bast technology, which is applied in the hemp bast degumming process and the field of enzyme preparations, can solve the problems of large degumming wastewater pollution, unstable quality, long degumming cycle, etc., and achieve short degumming time, rough culture conditions, and easy processing The effect of cost reduction

Active Publication Date: 2018-12-25
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an enzyme preparation that can be used for hemp degumming and the degumming process of hemp bast to solve the problems of long degumming cycle, unstable quality, large pollution of degumming wastewater and high cost in the existing degumming process

Method used

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  • Enzyme preparation that can be used for degumming of hemp and its bast degumming process of hemp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present invention can produce the strain of hemp bast degumming enzyme, the preservation number is CGMCC 14600, and the name is Dicardia chrysanthemum DC ( Dickeya chrysanthemi IBFC 2016), which was deposited in the China General Microorganism Collection Management Center on September 8, 2017.

[0031] The screening and identification method of the Dicardia chrysanthemum DC comprises the following steps:

[0032](1) Sampling and treatment: Take 10 g of the sludge sample from the retting pool, put it in a conical flask with an appropriate amount of glass beads, add 200 mL of sterile water, mix it at 180 rpm for 20 min, and filter it with multi-layer gauze to remove the residue Obtain a suspension.

[0033] (2) Enrichment screening: Mix 10 mL of suspension, 150 mL of tap water and 15 g of ramie bast, place in a Erlenmeyer flask, and ferment at 35°C with shaking at 180 rpm until the fibers are dispersed; take 10 mL of the fermentation broth, and 150 mL of tap water...

Embodiment 2

[0037] The Dicardia chrysanthemum DC obtained in Example 1 was applied, and on October 12-20, 2016, the preparation of the enzyme preparation and the degumming technology of apocynum apocynum were carried out in the Chinese Academy of Agricultural Sciences' hemp processing enzyme preparation pilot workshop. Process such as figure 1 As shown, the specific steps include:

[0038] (1) Preparation of enzyme preparation:

[0039] S1. Inoculate the DC strain of Dicarpus chrysanthemum into 5 mL of broth medium for activation, shake or stir culture at 34°C for 10 h, and then add it to a Erlenmeyer flask containing 200 mL of sterile broth culture medium cultured at 34°C with 180 rpm for 7 h to obtain a bacterial suspension;

[0040] S2. Transfer 2% of the inoculum to a fermenter containing sterile broth medium, stir and ferment at 34°C at a speed of 180 rpm, and feed compressed air into it. The flow rate of the soup medium is 0.2m 3 / (m 3 h), maintain a pressure of 0.3 MPa in the...

Embodiment 3

[0051] The Dicardia chrysanthemum DC obtained in Example 1 was applied, and on December 20-28, 2016, the preparation of enzyme preparations and the degumming of ramie were carried out in Hunan Lierkang Biological Company and Hanshou Ziyang Textile Raw Materials Co., Ltd. technology, the process of which is as figure 1 As shown, the specific steps include:

[0052] (1) Preparation of enzyme preparation:

[0053] S1. Inoculate the DC strain of Dicardia chrysanthemum into 5 mL broth medium for activation, shake or stir culture at 34°C for 12 h, and then add it to a Erlenmeyer flask containing 200 mL sterile broth culture medium , cultured with stirring at 200 rpm for 8 h at 34°C to obtain a bacterial suspension;

[0054] S2. Transfer 2% of the inoculum to a fermenter containing sterile broth medium, stir and ferment at 34°C at a speed of 200 rpm, and feed compressed air into it. The flow rate of the soup medium is 0.3m 3 / (m 3 h), maintain a pressure of 0.5 MPa in the ferme...

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Abstract

The invention discloses an enzyme preparation for hemp bast degumming. Xylanase and a CaCl2 assistant are added to a fermentation broth of Dickeya chrysanthemi, and above substances are mixed to obtain the enzyme preparation, wherein the preservation number of the is Dickeya chrysanthemi is CGMCC 14600, and the Dickeya chrysanthemi is named as Dickeya chrysanthemi DC. The invention also provides atechnology for degumming hemp bast by using the enzyme preparation. The technology sequentially comprises the following steps: preparing the enzyme preparation, preprocessing a hemp raw material, carrying out circulating immersion in the enzyme, performing inactivation, and washing hemp to finally obtain pure hemp fibers. The enzyme preparation has the advantages of rough culture conditions for the Dickeya chrysanthemi DC, high growth speed and high enzyme activity, and is suitable for to degumming of bast of ramie, kenaf, hemp, flax and kendir; and a circulating enzyme immersion process is adopted to cyclically use the enzyme preparation, so the use efficiency of the enzyme preparation is improved, the process flow is shortened, the production cost is reduced, pickling, high-pressure andhigh-concentration soda boiling and acid rinsing are not needed, conditions are mild, the treatment load of inorganic and organic pollutants is 70% less than that of chemical degumming, and the processing cost is reduced by about 20%.

Description

technical field [0001] The invention belongs to the technical field of hemp degumming, and in particular relates to an enzyme preparation which can be used for hemp degumming and a process for degumming hemp bast. Background technique [0002] The bast part of hemp crops such as ramie, kenaf, hemp, flax, and apocynum contains 20% to 40% of non-cellulose substances, including lignin, pectin, and hemicellulose, which are embedded between cells And in the cell wall, the structure is very complex, and the development and utilization of hemp needs to be extracted from fibers. The extraction process of fibers generally needs to remove non-fibrous substances, and the degumming process is the key step. [0003] Hemp degumming methods mainly include chemical degumming and biological degumming. The traditional retting method has problems such as long degumming cycle, serious pollution, difficult control of process conditions, and unstable fiber yield and quality; existing hemp degumm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24D01C1/00C12R1/01
CPCC12N9/248D01C1/00
Inventor 段盛文成莉凤彭源德冯湘沅郑科杨琦刘志远彭正红
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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