A kind of bast degumming method of apocynum bast
A degumming method and a technology of liquid culture medium are applied in the field of apocynum bast degumming to achieve the effects of small fiber damage, fast propagation speed and low energy consumption
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Embodiment 1
[0027] In the embodiment of the present invention, a kind of bast degumming method of apocynum bast comprises the following steps:
[0028] 1) Preparation of bacterial suspension: Take 1 ring of the preserved bacterial strain LM lawn and insert it into 5 mL of liquid medium, mix it and culture it at 34 °C and 180 r / min for 6 h to prepare culture A; take 1 mL for culture Bacteria A was diluted and spread on solid medium, cultured at 34 °C for 20 h, and culture B was obtained; an activated colony was picked from culture B and inserted into 5 mL of liquid medium, and cultured at 34 °C, 180r / min Cultivate under the condition of 4 h to obtain the culture C; pour the culture C into 100 mL liquid medium, and cultivate it at 34 °C and 180 r / min for 5 h to obtain the culture D; take 6 mL of the culture D was inoculated with 300 mL of liquid medium, and cultured at 34 °C and 180 r / min for 6 h to obtain a bacterial suspension. Wherein the liquid medium is composed of the following compo...
Embodiment 2
[0032] In the embodiment of the present invention, a kind of bast degumming method of apocynum bast comprises the following steps:
[0033]1) Preparation of bacterial suspension: Take 1 ring of the preserved bacterial strain LM lawn and insert it into 5 mL of liquid medium, mix it and culture it at 34 °C and 180 r / min for 6 h to prepare culture A; take 1 mL for culture Bacteria A was diluted and spread on solid medium, cultured at 34 °C for 20 h, and culture B was obtained; an activated colony was picked from culture B and inserted into 5 mL of liquid medium, and cultured at 34 °C, 180r / min Cultivate under the conditions of 4 h to obtain culture C; pour culture C into 100 mL of liquid medium, and cultivate at 34 °C and 180 r / min for 5 h to obtain culture D; take 6 mL of culture Bacteria D was inoculated with 300 mL of liquid medium, and cultured at 34 °C and 180 r / min for 6 h to obtain a bacterial suspension. Wherein the liquid medium is composed of the following components a...
Embodiment 3
[0037] In the embodiment of the present invention, a kind of bast degumming method of apocynum bast comprises the following steps:
[0038] 1) Preparation of bacterial suspension: Take 1 ring of the preserved bacterial strain LM lawn and insert it into 5 mL of liquid medium, mix it and culture it at 34 °C and 180 r / min for 6 h to prepare culture A; take 1 mL for culture Bacteria A was diluted and spread on solid medium, and cultured at 34 °C for 20 h to obtain culture B; one activated colony was picked from culture B and inserted into 5 mL of liquid medium, and cultured at 34 °C, 180 r / min under the condition of culturing for 4 h to obtain culture C; pour culture C into 100 mL of liquid medium, and incubate at 34 °C and 180 r / min for 5 h to obtain culture D; take 6 Inoculate 300 mL of liquid culture medium with mL of culture D, and culture at 34°C and 180 r / min for 6 h to obtain a bacterial suspension. Wherein the liquid medium is composed of the following components accordi...
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