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Enzyme preparation for bast fiber degumming, and hemp bast degumming technology thereof

An enzyme preparation and bast technology, applied in the field of hemp bast degumming process and enzyme preparation, can solve the problems of large pollution of degumming wastewater, unstable quality, long degumming cycle, etc., and achieve rough culture conditions, wide application range, and processing. cost reduction effect

Active Publication Date: 2018-03-23
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an enzyme preparation that can be used for hemp degumming and the degumming process of hemp bast to solve the problems of long degumming cycle, unstable quality, large pollution of degumming wastewater and high cost in the existing degumming process

Method used

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  • Enzyme preparation for bast fiber degumming, and hemp bast degumming technology thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The strain of the present invention that can produce hemp bast degumming enzyme, the preservation number is CGMCC 14600, named as Dickeya chrysanthemi IBFC 2016, which was preserved in China's common microbial strains on September 08, 2017 Preservation Management Center.

[0033] The screening and identification method of the Dicardia chrysanthemum DC comprises the following steps:

[0034] (1) Sampling and treatment: Take 10 g of the sludge sample from the retting pool, place it in a conical flask with an appropriate amount of glass beads, add 200 mL of sterile water, mix at 180 rpm for 20 min, filter and remove the slag with multi-layer gauze to obtain a suspension.

[0035] (2) Enrichment screening: take 10mL of suspension, 150mL of tap water and 15g of ramie bast, mix them in a conical flask, and ferment at 35°C with shaking at 180rpm until the fibers are dispersed; take 10mL of the fermentation broth, 150mL of tap water, and 15g of cannabis Mix bast, place in coni...

Embodiment 2

[0039] The Dicardia chrysanthemum DC obtained in Example 1 was applied, and on October 12-20, 2016, the preparation of the enzyme preparation and the degumming technology of apocynum apocynum were carried out in the Chinese Academy of Agricultural Sciences' hemp processing enzyme preparation pilot workshop. Process such as figure 1 As shown, the specific steps include:

[0040] (1) Preparation of enzyme preparation:

[0041] S1. Inoculate the DC strain of Dicarpus chrysanthemum into 5mL broth medium for activation, shake or stir culture at 34°C for 10h, then add it to a Erlenmeyer flask containing 200mL sterile broth culture medium, and Stirring culture at 180rpm at 34°C for 7h to obtain bacterial suspension;

[0042] S2. transfer to the fermentor containing aseptic broth medium by 2% inoculum size, stir and ferment with the rotating speed of 180rpm at 34 ℃, and pass into compressed air wherein, compressed air is to containing aseptic broth The flow rate of the culture medi...

Embodiment 3

[0053] The Dicardia chrysanthemum DC obtained in Example 1 was applied, and on December 20-28, 2016, the preparation of enzyme preparations and the degumming of ramie were carried out in Hunan Lierkang Biological Company and Hanshou Ziyang Textile Raw Materials Co., Ltd. technology, the process of which is as figure 1 As shown, the specific steps include:

[0054] (1) Preparation of enzyme preparation:

[0055] S1. Inoculate the DC strain of Dicardia chrysanthemum into 5mL broth medium for activation, shake or stir culture at 34°C for 12h, then add it to a Erlenmeyer flask containing 200mL sterile broth culture medium, and Stirring culture at 200rpm at 34°C for 8h to obtain bacterial suspension;

[0056] S2. transfer to the fermenter containing aseptic broth medium by 2% inoculum size, stir and ferment with the rotating speed of 200rpm at 34 ℃, and pass into compressed air wherein, compressed air is to containing aseptic broth The flow rate of the culture medium is 0.3m 3 ...

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Abstract

The invention discloses an enzyme preparation for hemp bast degumming. Xylanase and a CaCl2 assistant are added to a fermentation broth of Dickeya chrysanthemi, and above substances are mixed to obtain the enzyme preparation, wherein the preservation number of the is Dickeya chrysanthemi is CGMCC 14600, and the Dickeya chrysanthemi is named as Dickeya chrysanthemi DC. The invention also provides atechnology for degumming hemp bast by using the enzyme preparation. The technology sequentially comprises the following steps: preparing the enzyme preparation, preprocessing a hemp raw material, carrying out circulating immersion in the enzyme, performing inactivation, and washing hemp to finally obtain pure hemp fibers. The enzyme preparation has the advantages of rough culture conditions for the Dickeya chrysanthemi DC, high growth speed and high enzyme activity, and is suitable for to degumming of bast of ramie, kenaf, hemp, flax and kendir; and a circulating enzyme immersion process is adopted to cyclically use the enzyme preparation, so the use efficiency of the enzyme preparation is improved, the process flow is shortened, the production cost is reduced, pickling, high-pressure andhigh-concentration soda boiling and acid rinsing are not needed, conditions are mild, the treatment load of inorganic and organic pollutants is 70% less than that of chemical degumming, and the processing cost is reduced by about 20%.

Description

technical field [0001] The invention belongs to the technical field of hemp degumming, and in particular relates to an enzyme preparation which can be used for hemp degumming and a process for degumming hemp bast. Background technique [0002] The bast part of hemp crops such as ramie, kenaf, hemp, flax, and apocynum contains 20% to 40% of non-cellulose substances, including lignin, pectin, and hemicellulose, which are embedded between cells And in the cell wall, the structure is very complex, and the development and utilization of hemp needs to be extracted from fibers. The extraction process of fibers generally needs to remove non-fibrous substances, and the degumming process is the key step. [0003] Hemp degumming methods mainly include chemical degumming and biological degumming. The traditional retting method has problems such as long degumming cycle, serious pollution, difficult control of process conditions, and unstable fiber yield and quality; existing hemp degumm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/24D01C1/00C12R1/01
CPCC12N9/248D01C1/00
Inventor 段盛文成莉凤彭源德冯湘沅郑科杨琦刘志远彭正红
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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