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Rapid and mass spore production method for sweet potato black rot bacteria

A technology for black spot fungus and black spot disease, which is applied in the field of agricultural plant protection, can solve the problems of low efficiency and long time consumption, and achieves the effects of scientific and rapid method, improved spore production and simple process.

Inactive Publication Date: 2018-03-20
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of washing and filtering spores from black spot plates is time-consuming and inefficient, and cannot quickly and massively provide the conidia suspensions required for tests such as drug screening and drug resistance risk assessment.

Method used

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  • Rapid and mass spore production method for sweet potato black rot bacteria
  • Rapid and mass spore production method for sweet potato black rot bacteria
  • Rapid and mass spore production method for sweet potato black rot bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A kind of method for fast large-scale spore production of sweet potato black spot fungus specifically comprises the following steps:

[0026] 1) Isolation and purification of black spot bacteria: collect black spot diseased potato pieces from sweet potato cellars, and isolate black spot bacteria by tissue separation method; first clean the surface of the potato pieces, then rinse them with running water for about 20 minutes, and remove the diseased potato pieces. Take it out and dry it and put it in an ultra-clean workbench, put the disease-healthy junction of black spot disease into 75% alcohol for surface disinfection, then disinfect it with 0.1% mercuric chloride, clean it with sterilized water, and place it on potato dextrose agar (PDA) plate culture, after separation and purification and Koch's rule validation confirmed to retain the strain;

[0027] 2) Cultivation of black spot bacteria and preparation of spore suspension:

[0028] Control group 1: Use PDA plates...

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Abstract

The invention discloses a rapid and mass spore production method for sweet potato black rot bacteria. The method comprises the steps of collecting diseased sweet potato blocks, separating black rot bacteria by virtue of a tissue separation method, inoculating the black rot bacteria into a PS liquid culture medium, carrying out shaking culture at temperature of 31 DEG C and a rotation speed of 120rpm / min for 24 hours, carrying out gauze filtration so as to remove hyphae, carrying out microscopic examination to determine the concentration of spore suspension liquid, and adjusting the concentration of the spore suspension liquid through sterile water to meet the test requirement. The rapid and mass spore production method is scientific, efficient and simple to operate, the preparation time oftraditional black rot bacteria spore suspension liquid can be greatly shortened, the pollution of infectious microbes in a traditional spore suspension liquid preparation method is avoided, and the powerful guarantee is provided for smooth execution of the accurate resistance identification of the sweet potato black rot bacteria, the screening of indoor medicaments of the black rot bacteria, thedrug resistance risk evolution and other tests needing to be finished by virtue of conidium suspension liquid.

Description

technical field [0001] The invention relates to a method for rapid and massive sporulation of sweet potato black spot fungus, which belongs to the field of agricultural plant protection. Background technique [0002] Sweet potato black spot (sweeppotato black rot) is one of the main diseases in the cultivation and storage of sweet potatoes. It occurs in all sweet potato producing areas in the world. The pathogen is Ceratocystis fimbriata Ellis et Halsted, which is widely distributed. , The population differentiation is complex, mainly harming the stem base and root tuber tissue of seedlings, causing dead seedlings, rotten beds, and rotten cellars, which have a great impact on sweet potato production. The diseased potato with black spot fungus contains substances such as sweet potato scleromycin, which can cause poisoning symptoms after consumption by livestock; using diseased potato as fermentation raw material will delay the fermentation process, reduce alcohol production a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 王波周兴根杨冬静黄忠勤孙克新丁震乾常勇李小珊周涧楠苏在兴
Owner XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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