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Preparation method of ganoderma sinense culture medium

A culture medium and technology of Zizhi, applied in the preparation of organic fertilizers, botanical equipment and methods, cultivation and other directions, can solve the problems of many types of Ganoderma lucidum, high cost, and many raw material processing procedures, and achieve a simple production process and low production cost. , improve the effect of comprehensive utilization

Inactive Publication Date: 2018-03-20
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two inventions all use more than 6 kinds of raw materials, and then prepare the Ganoderma lucidum culture medium through conventional techniques. However, due to the variety of raw materials, there are many raw material processing procedures and high costs, and there are many types of Ganoderma lucidum. Applicable to all ganoderma strains, so if different ganoderma strains are cultivated, it is necessary to re-explore the proportion of raw materials used through a large number of experiments

Method used

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  • Preparation method of ganoderma sinense culture medium
  • Preparation method of ganoderma sinense culture medium
  • Preparation method of ganoderma sinense culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Take 60kg of wood chips, 35kg of olive pomace obtained after olive fruit oil extraction, and 60kg of drinking water, mix well, let stand for moistening for 25min, move to a steamer and cook for 25min under normal pressure;

[0025] 2) Cool the mixed material cooked in step 1) to 35°C, add 6kg of Aspergillus oryzae bran koji, mix evenly, keep fermenting at 30°C for 38 hours, after the fermentation, add 40kg of drinking water at 50°C, and heat-preserve at 50°C for enzymatic hydrolysis 32h;

[0026] 3) The mixed material after step 2) enzymolysis is finished, bagged: 0.5kg per bag, bag diameter 10cm, bag height 15cm; steam sterilization at normal pressure for 20min, and the preparation of Zizhi culture medium can be directly inoculated with Zizhi. Used to cultivate Zizhi fruiting bodies.

Embodiment 2

[0027] Embodiment 2, with embodiment 1, difference is,

[0028] 1) The amount of drinking water is 75kg, let stand to moisten the water for 38 minutes, move it to a steamer and steam for 33 minutes under normal pressure;

[0029] 2) adding Aspergillus oryzae bran koji for 42 hours of heat preservation and fermentation, and 39 hours of heat preservation and enzymolysis at 54°C.

[0030] Compared with Example 1, the amount and time of wetting water have been increased, and the fermentation and enzymolysis time of Aspergillus oryzae have been prolonged, so that the content of small molecular substances, B vitamins and inorganic salts in the culture medium is greater.

Embodiment 3

[0032] 1) Take 90kg of wood chips, 60kg of olive pomace obtained after olive fruit oil extraction, and 80kg of drinking water, mix them uniformly, let stand to moisten the water for 40min, move to a steamer and cook for 35min under normal pressure;

[0033] 2) Cool the mixed material cooked in step 1) to 35°C, add 10kg of Aspergillus oryzae bran koji, mix evenly, keep fermenting at 33°C for 44 hours, after the fermentation, add 47kg of drinking water at 50°C, and heat-preserve at 55°C for enzymatic hydrolysis 40h;

[0034] 3) The mixed material after step 2) enzymatic hydrolysis is finished, packed into bags: 0.5 kg of material in each bag, with a bag diameter of 10 cm and a bag height of 15 cm; sterilized by atmospheric pressure steam for 20 minutes to prepare a Zizhi culture medium.

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Abstract

The invention discloses a preparation method of a ganoderma sinense culture medium. The method solves the problem that the existing olive tree branches and fruit slag are not effectively utilized. Thepreparation method comprises the following steps of (1) trimmed olive tree branches are dried and are then crushed by a tree branch crushing machine to obtain wood chips; olive fruit are subjected tooil extraction to obtain olive fruit slag; the wood chips, the olive fruit slag and drinking water are mixed and are then steamed and boiled; (2) a mixture steamed and boiled in the step (1) is fermented after the aspergillus oryzae mouldy bran addition; then, drinking water is added; heat insulation enzymolysis is performed; (3) after the enzymolysis in the step (2) is completed, the mixed materials are subjected to bag charging and sterilization to prepare the ganoderma sinense culture medium. The method has the advantages that the comprehensive utilization rate of the olive tree can be improved; the product added value is improved; the operability is high; the preparation method is very suitable for culturing the ganoderma sinense.

Description

technical field [0001] The invention belongs to the technical field of edible fungus production, and in particular relates to a preparation method of a purple lucidum culture medium. Background technique [0002] Olive oil, a world-famous woody oil plant, has high edible value. Olive fruit is rich in high-quality edible vegetable oil——olive oil, which is recognized by the European Union as a high-grade vegetable oil that is beneficial to the body. It contains carotenoids, omega-6 Fatty acids, omega-3 fatty acids, squalene, olive oil and isoleatin, multivitamins and unsaturated fatty acids. The olive pomace after oil extraction contains nutrients such as starch, protein, cellulose, lipid, minerals, and carotenoids. At present, the olive pomace is directly discarded, causing a great waste of resources, and the olive branches trimmed from the olive tree are also directly discarded, and are burned to cause air pollution. Deeply processed products of olive branches and olive po...

Claims

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Application Information

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IPC IPC(8): C05F15/00C05F17/00A01G18/20
CPCC05F5/002C05F17/00C05F11/00C05F11/08Y02P20/133Y02W30/40
Inventor 柳志杰高冰祁勇刚李冬生汪超胡勇徐宁周梦舟吴茜
Owner HUBEI UNIV OF TECH
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