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Manual scaled feeding method for diglyphus wani Liu, Zhu & yefremova sp. nov.

A technology for larvae and larvae, which is applied to the field of artificial large-scale rearing of the larvae of Liriomyza wanyi, and can solve the problems of different optimal conditions for establishment of parasitic wasp populations, the repeatability of feeding methods to be improved, and the inconvenient use of rearing equipment, etc. problems, to achieve the effects of easy control of host density, increased spawning parasitic rate, and convenient and fast feeding.

Inactive Publication Date: 2018-03-20
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0006] At present, the main technical problems in the artificial breeding of parasitoids are concentrated in the high cost of breeding, different optimization conditions for the establishment of parasitoid populations, inconvenient use of breeding utensils, the repeatability of breeding methods needs to be improved, the realization of rapid population expansion methods, and the full use of biological control methods. conditions of potential

Method used

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  • Manual scaled feeding method for diglyphus wani Liu, Zhu & yefremova sp. nov.
  • Manual scaled feeding method for diglyphus wani Liu, Zhu & yefremova sp. nov.
  • Manual scaled feeding method for diglyphus wani Liu, Zhu & yefremova sp. nov.

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Experimental program
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Effect test

Embodiment 1

[0028] The artificial large-scale breeding method of Liriomyza wanneri comprises the following steps:

[0029] (1) Selection of milk pinto plants as host plants: 11 kinds of host plants were selected, including British red kidney beans, milk pinto beans, lettuce, rapeseed, Shanghai green, broad beans, collard greens, green leafy cabbage, kale, and peas. Liriomyza pea was raised under humidity conditions, but by comparison, lettuce leaves were the most effective for indoor breeding to establish populations, followed by rapeseed, Shanghai green, Chinese kale, British red kidney beans, and milk pinto beans. However, during the breeding process, each leaf of lettuce only has a density of 20 host larvae at most, and the growth cycle of lettuce, rapeseed, Shanghai green, kale, and cabbage is relatively long, and it usually takes about 2 months to reach a usable state. And planting method is loaded down with trivial details, and seedling production cost is higher. In addition, the d...

Embodiment 2

[0038] Potential of controlling Liriomyza sativae to Liriomyza sativae under different temperatures in embodiment 2

[0039] Prepare in advance the milky bean leaf petri dish containing 30-35 late 2nd instar to early 3rd instar host larvae of Liriomyza sativae. Production of host food petri dishes: 1) Select about 30 leaves of milk bean containing about 30 late 2nd and 3rd instar larvae of Liriomyza sativae; 2) Make agar (Coolaber TM , Cat#CA1331-1KG); 3) Pour the agar into the petri dish, cut off the leaves that contain about 30 leaves of Liriomyza sativae larvae from the end of the 2nd instar to the early stage of the 3rd instar, and insert the petioles into the agar. Gently place on the agar; 4) Seal the seal with a parafilm, and pierce holes in the parafilm with a dissecting needle; 5) Place the prepared culture dish in an artificial climate incubator (temperature T=25±1°C, relative humidity RH= 30±5%, photoperiod L:D=14:10).

[0040] Select the Liriomyza wanshii wasps t...

example 3

[0044] Example 3 The Potential of Liriomyza wansonii to control Liriomyza sativae under different nutrition

[0045] From the beekeeping cages in the artificial climate incubator (temperature T = 25 ± 1 ° C, relative humidity RH = 50 ± 5%, light cycle L: D = 14:10), select the first eclosion at 8:00-10:00 in the morning wan's Liriomyza spp. Provide 4 treatments: 20% glucose + host larvae, 20% honey water (acacia honey) + host larvae, 20% glucose + 10% yeast + host larvae, distilled water + host larvae, and the provided host larvae are about 30 At the end of the 2nd instar - the beginning of the 3rd instar, Liriomyza sativae larvae are used as food; at the same time, it is necessary to detect the lifespan of parasitoids that only provide 20% glucose, 20% honey water, 20% glucose + 10% yeast, distilled water, and then put them all in In an artificial climate chamber (temperature T=25±1°C, relative humidity RH=50±5%, photoperiod L:D=14:10), experiments were carried out on the in...

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Abstract

The invention relates to the field of parasitic wasp feeding, in particular to a manual scaled feeding method for diglyphus wani Liu, Zhu & yefremova sp. nov.. The method comprises the steps that hostplants, namely light speckled kidney bean, are planted; hosts, namely liriomyza sativae blanchard, is fed; light speckled kidney bean seedling leaves each containing 30-35 liriomyza sativae blanchardhost larvae are supplied to the diglyphus wani Liu, Zhu & yefremova sp. nov. for feeding and oviposition at the room temperature of 25 DEGC. The method is convenient and fast to use, the production cost is low, it can be achieved that the propagation of diglyphus wani Liu, Zhu & yefremova sp. nov. is expanded on a large scale, and then the diglyphus wani Liu, Zhu & yefremova sp. nov. is releasedin the area where leaf miner pests are serious, chemical pesticides can be effectively reduced in use, the pollution of the pesticides to the environment is reduced, food safety is ensured, and greateconomic, ecological and social benefits are brought.

Description

technical field [0001] The invention relates to the field of breeding of parasitoid bees, in particular to an artificial large-scale breeding method for Liriomyza wannerii. Background technique [0002] Agromyzid leafminers (agromyzid leafminers) are a class of worldwide important small pests that harm economic crops such as vegetables and flowers. Reasonable use of parasitic wasps for biological control has become the preferred strategy for the prevention and control of leafminer pests. [0003] A dominant parasitic wasp of the leaf miner, Diglyphus isaea (Walker), is prone to sex ratio imbalance (male bias) in the offspring during the breeding process, which leads to difficulties in continuous breeding and high production costs. Leafminer is a parthenogenetic reproduction model. It does not need to mate with a male to produce females. All offspring are females. There is no situation where the population is difficult to multiply. In practical applications, it is mainly the ...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K20/163A23K10/20
CPCA01K67/033A23K10/20A23K20/163A23K50/90
Inventor 刘万学叶福宇轩景丽潘立婷杜素洁郭建洋
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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