Method for detecting influence of electronic cigarette aerosol water extract on activity of superoxide dismutase of cells

Abstract

The invention discloses a method for detecting the influence of an electronic cigarette aerosol water extract on the activity of superoxide dismutase of cells. The method comprises: sample pre-treatment, single cell suspension preparation, cell concentration calculation, cell inoculation, subject exposure, cell collection, cell lysis, sample determination, superoxide dismutase enzyme activity calculation, and other steps. According to the present invention, the expose way and the action mode of the electronic cigarette product are comprehensively inspected, the completely-new electronic cigarette product smoke pre-treatment method is established, human lung fibroblasts HPF are selected as detection cells, and the sample detection dose is determined; and through the effective sample treatment, the detection dose optimization and the correct target cell selection, the method can accurately determine the influence of the electronic cigarette aerosol water extract on the activity of the superoxide dismutase of the cells, such that the influence of the electronic cigarette product on the oxidative damage of cells can be effectively evaluated.

Description

technical field [0001] The invention belongs to the technical field of safety biological evaluation of tobacco and tobacco products, and in particular relates to a method for detecting the influence of electronic cigarette aerosol water extract on cell superoxide dismutase activity. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. E-cigarette products avoid the harm caused by the complex mixture produced by the combustion of traditional cigarette products. At this stage, it has become one of the new directions for foreign tobacco companies to shift from traditional cigarette products. During the use of electronic cigarette products, the liquid composed of nicotine, flavoring substances, etc. is converted into a mixture similar to cigarette smoke by electric heating or electronic...

AI Technical Summary

Problems solved by technology

However, the existing technology usually directly analyzes and tests the e-cigarette liquid itself, without considering its shape, migration and other processes during smoking, which leads to errors in the results of the analysis and test, and cannot accurately reflect the disproportionation of cell superoxide by e-cigarette liquid. The Real Impact of Enzyme Activity

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