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Vibrio parahaemolyticus phage and bactericidal composition containing the same

A technology of Vibrio hemolyticus and bacteriophage, applied in the direction of bacteriophage, virus/phage, medical raw materials derived from virus/phage, etc., can solve the problems of narrow host spectrum, short duration of lysis ability, and easy resistance of host bacteria.

Pending Publication Date: 2018-03-16
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the important weapons to deal with antibiotic resistance, phage has great potential for clinical application, but as far as its current application of single phage therapy is concerned, there are still some potential disadvantages in the application process, such as narrow lytic host spectrum and lytic ability. The duration is short, and the host bacteria are easy to develop resistance, etc. Among them, the host bacteria are easy to develop resistance as the main defect in the application of single phage

Method used

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  • Vibrio parahaemolyticus phage and bactericidal composition containing the same
  • Vibrio parahaemolyticus phage and bactericidal composition containing the same
  • Vibrio parahaemolyticus phage and bactericidal composition containing the same

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Embodiment

[0092] The present invention separates and obtains 6 strains of pathogenic Vibrio from its focus by collecting sick fish and shrimp samples from aquaculture plants, and the screening flow chart of phage of the present invention is as follows figure 1 shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and 5 of them were determined to be extremely pathogenic, LD 50 4 CFU / mL, action time figure 2 shown.

[0093] Through the plate spot method, according to the presence or absence of transparent spots, the host spectrum of the Vibrio phage under 29 strains of different genus was measured (Table 1), and it was concluded that the phage had strong host specificity and was a Vibrio phage. Is a specific split phage.

[0094] Table 1 host spectrum

[0095]

[0096]

[0097]

[0098] Remarks: "+" means it can lyse, "-" means it can't lyse; the above strains are all isolated from aquaculture fish and shrimp animals and aquaculture water; ...

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Abstract

The invention relates to a vibrio parahaemolyticus phage and a bactericidal composition containing the same. The phage can rapidly inhibit the growth of vibrios in a short time, and the duration of inhibition lasts long. In addition, the bactericidal composition of the phage is safe and effective, has strong specificity, is low in production cost, and makes up for the shortcoming of single phagotherapy in the control process of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Vibrio parahaemolyticus phage and a bactericidal composition containing the phage. Background technique [0002] my country is a big country in aquaculture. In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the continuous promotion of intensive farming methods, the frequent occurrence of fish diseases poses a serious threat to the healthy development of large-scale marine fish farming. Fish pathogens mainly include bacteria, viruses, parasites, etc. Among them, bacterial diseases are a type of disease that is more harmful to fish. [0003] Vibrio is a short rod-shaped spore-shaped bacterium that can move. It is widely distributed in seawater and organisms in coastal and estuary sea areas. It is one of the most common bacterial groups in the ocean, and it is also the most important pathogenic bacteria that...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76C12N7/00C12N2795/00021C12N2795/00032Y02A50/30
Inventor 马迎飞陈玲樊继强
Owner SHENZHEN INST OF ADVANCED TECH
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