Electrospray ionization mass spectrometry interface device with tiny dead volume and utilization method thereof

Abstract

The invention discloses an electrospray ionization mass spectrometry interface device with tiny dead volume and a utilization method thereof. The interface device comprises an analysis capillary tube,a sheath flow capillary tube, a sheath flow connection tube, a connection component and an electrode. A sheath flow solution storage container and the sheath flow capillary tube are connected throughthe sheath flow connection tube, the connection component is used for assembling the analysis capillary tube and the sheath flow capillary tube into a sleeve structure, and the electrode is used forproviding power for electrospray. The interface device has advantages that by design of processing the thin-wall analysis capillary tube, the small-outlet sheath flow capillary tube, laminar sheath fluid surface flow and the like, the interface dead volume can be sharply decreased, sample separation efficiency is guaranteed, and the service life of the interface device is prolonged.

Description

Technical field: [0001] The field of the invention relates to the field of mass spectrometry in analytical chemistry, in particular to an electrospray ionization mass spectrometry interface with a small dead volume and a use method thereof. Background technique: [0002] Mass spectrometry is playing an increasingly important role in the development of the current field of analytical chemistry. The electrospray ionization mass spectrometry system coupled with high-resolution separation systems such as capillary electrophoresis or liquid chromatography has unique advantages in the analysis of complex samples containing proteins, polypeptides, nucleic acids, and small molecules. The interface structure and characteristics of the separation analysis system and the electrospray mass spectrometry system will significantly affect the analysis performance of the coupled system. First, reducing the dead volume of the interface can reduce the dilution effect of the sample after separ...

AI Technical Summary

Problems solved by technology

This type of capillary-based electrospray ionization mass spectrometry interface has the advantages of simple fabrication, convenience, and low cost, but there are also some obvious limitations

For example, there is a certain space between the outlet of the analytical capillary and the outlet of the sheath flow sleeve in this type of interface. The dead volume formed in this space will cause the sample to diffuse here after separation, resulting in a decrease in separation efficiency.

Secondly, the solution in the sheath flow cannula is focused in the tip of the sheath flow cannula, which will easily cause the nozzle to be blocked, so that the electrospray stops and the experiment is interrupted.

In addition, the outer diameter of the analysis capillary is larger than the inner diameter of the sheath flow sleeve, which may easily cause collision between the analysis capillary and the sheath flow sleeve during the installation and use of the interface, thereby damaging the analysis capillary or the sheath flow sleeve and reducing the service life of the interface

Although it has been reported to use a sheath flow sleeve with a larger inner diameter to solve the problems of nozzle clogging and service life, the dead volume of the interface is still large in this method, which reduces the separation efficiency and detection sensitivity of the sample.

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