BRAF gene mutation detection system and kit thereof

Abstract

The invention relates to a BRAF gene mutation detection system and a kit thereof. The kit comprises a PCR detection liquid and SYBR GreenI mixed liquor, wherein the PCR detection liquid comprises forward and reverse primers and peptide nucleic acid which are used for detecting a site V60OE of a 600th codon of a BRAF gene; and a nucleotide sequence of the peptide nucleic acid is completely complementary with the site V60OE of the 600th codon of a BRAF wild-type gene. The BRAF gene mutation detection system and the kit thereof have high sensitivity and specificity, demanded sample quantity is low, and BRAF gene mutation detection can be quickly, conveniently and accurately carried out.

Description

technical field [0001] The invention relates to a method for detecting BRAF gene mutation and detection products, belonging to the field of biotechnology. Background technique [0002] The BRAF gene is located on chromosome 7q34 and is one of the most important proto-oncogenes in humans. It encodes a serine / threonine kinase and is an important transduction factor in the EGFR pathway RAS / RAF / MEK / MRK / MAPK and participates in the regulation of cell growth , differentiation and apoptosis and other physiological processes. Studies have shown that there are different rates of BRAF gene mutations in various malignant tumors such as malignant melanoma, lung cancer, and colorectal cancer, among which about 82% of the mutations are located at the 1799th nucleotide of exon 15, and the T mutation is A (T1799A ), causing the glutamic acid encoded by it to be replaced by valine (V600E). This mutation activates the BRAF protein and leads to the continuous activation of the downstream MEK-...

AI Technical Summary

Problems solved by technology

[0004] At present, technologies such as next-generation sequencing, FLP, allele-specific oligonucleotide hybridization, allele-specific PCR, and gene chips are mainly used to detect human BRAF gene mutations. , often requires electrophoresis and multi-step post-processing, which affects the accuracy of the test results

Or the instruments and equipment are expensive, and it is difficult to promote and apply them on a large scale

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