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Blood lipid reducing oligo-peptides of miichthys miiuy flesh

A technology of squid fish meat and defatted squid fish meat, which is applied in the direction of specific peptides, connective tissue peptides, animal/human proteins, etc., to achieve the effect of promoting β-oxidation, high yield, and good lipid-lowering activity

Inactive Publication Date: 2018-03-06
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of blood lipid-lowering peptides by enzymatic hydrolysis using squid meat as raw material

Method used

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  • Blood lipid reducing oligo-peptides of miichthys miiuy flesh
  • Blood lipid reducing oligo-peptides of miichthys miiuy flesh
  • Blood lipid reducing oligo-peptides of miichthys miiuy flesh

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Experimental program
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Effect test

Embodiment 1

[0037] A blood lipid-lowering oligopeptide of squid fish meat, its preparation process is as follows: squid fish meat "defatting" enzymatic hydrolysis, enzymatic hydrolyzate, ultrafiltration, macroporous resin purification, ion exchange chromatography, gel filtration chromatography, high efficiency Functional evaluation of "hypolipidemic oligopeptide" prepared by liquid chromatography.

[0038] A blood lipid-lowering oligopeptide of squid meat, the specific steps of its preparation method are:

[0039] 1) Pretreatment of squid meat: remove the skin and bones of the squid, homogenate the fish meat until it becomes a paste, add ethyl acetate solution according to the ratio of solid to liquid 1g:5mL, stir and degrease for 24 hours, filter, and dry to obtain defatted squid Fish meat.

[0040] 2) Enzymatic hydrolysis of squid meat: take defatted squid meat, add distilled water according to the ratio of material to liquid 1g:5mL, adjust the pH value to 7.0, add papain at 1.0% of th...

Embodiment 2

[0049] A kind of hypolipidemic oligopeptide of squid fish meat, its preparation method specifically comprises the following steps:

[0050] 1) Pretreatment of squid meat: remove the skin and bones of the squid, homogenize the fish meat to a paste, add ethyl acetate solution according to the ratio of solid to liquid 1g:4mL, stir and degrease for 22 hours, filter, and dry to obtain defatted squid fish meat;

[0051] 2) Enzymatic hydrolysis of squid meat: Take defatted squid meat, add distilled water according to the ratio of material to liquid 1g:6mL, adjust the pH value to 7.2, add papain at 1.3% of fish meat weight, enzymatically hydrolyze at 48°C for 4h, and carry out at 92°C Inactivate the enzyme for 12 minutes; when the temperature of the enzyme solution is lowered to 55°C, adjust the pH value to 9.0, add 1.6% alkaline protease by weight of fish meat, react for 3.7 hours, inactivate the enzyme at 93°C for 11 minutes, cool to room temperature, centrifuge at 12,000rmp for 19 ...

Embodiment 3

[0061] A kind of hypolipidemic oligopeptide of squid fish meat, its preparation method specifically comprises the following steps:

[0062] 1) Pretreatment of squid meat: remove the skin and bones of the squid, homogenate the fish until it becomes a paste, add ethyl acetate solution according to the ratio of material to liquid 1g:5.5mL, and then add 0.003% dodecyl by weight of the fish Sodium sulfate, stirred and degreased for 25 h, filtered and dried to obtain defatted squid meat. The fat removal rate in the fish meat under this step is high, not only can remove non-collagen miscellaneous proteins, but also the loss of collagen is small, and has a small effect on fish meat. The performance of other ingredients has no effect, and the added sodium lauryl sulfate has a synergistic effect with ethyl acetate, which can increase the solubility of fat in ethyl acetate, improve the degreasing efficiency, and reduce the impact on the performance of collagen, improve non-toxicity The r...

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Abstract

The invention provides blood lipid reducing oligo-peptides of miichthys miiuy flesh. A preparation method specifically comprises the following steps: performing flesh defatting and enzymatic hydrolysis with a compound enzyme on the miichthys miiuy flesh taken as a raw material to obtain enzymatic hydrolysate, and performing ultrafiltration, desalting with macroporous resin, chromatography with anion exchange resin, gel column chromatography and reversed-phase high-performance liquid chromatography separation and purification on the enzymatic hydrolysate to obtain the blood lipid reducing oligo-peptides Val-Ile-Ala-Pro-Trp (VIAPW), the molecular weight being measured to be 584.72Da by ESI-MS (electrospray ionization mass spectrometry). By the VIAPW, adipogenesis genes can be reduced, oxidation of fatty acid beta can also be promoted, generation of lipid is reduced, LXRalpha and LXRbeta are activated, the blood lipid reducing level is higher than the lipid generation capability, and higher blood lipid reducing activity is achieved. The VIAPW has the advantages of safe, no toxic or side effects, high blood lipid reducing activity and the like, and can be developed as a blood lipid reducing medicament and health food.

Description

technical field [0001] The invention relates to a blood lipid-lowering oligopeptide, in particular to a blood-lipid-lowering oligopeptide of squid meat. Background technique [0002] Blood lipid is a general term for neutral fats such as triglycerides and cholesterol, and lipids such as glycolipids and steroids in plasma. Hyperlipidemia is caused by abnormal blood lipid metabolism in the body. Cardiovascular and cerebrovascular diseases such as atherosclerosis, coronary heart disease, and myocardial infarction induced by hyperlipidemia have seriously threatened human health. With the continuous improvement of modern people's living standards and changes in lifestyle, the number of patients with hyperlipidemia due to poor diet structure and unhealthy living habits is increasing day by day, and is developing towards a younger trend. Therefore, the related research on the screening of blood lipid-lowering functional components and the development of blood lipid-lowering drugs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16A61P3/06
CPCC12P21/06C07K14/78
Inventor 王斌潘欣陶静赵玉勤
Owner ZHEJIANG OCEAN UNIV
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