Application of mixed preparation of 5-aminolevulinic acid and forchlorfenuron to Leymus chinensis Tzvel planting
A technology of aminolevulinic acid and forchlorfenuron, which is applied in the field of plant growth, can solve the problems of limiting regulator potential, cell division lag, and plant growth speed can not keep up, so as to promote dry matter accumulation, enhance photosynthetic rate, improve The effect of plant stress resistance
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Embodiment 1
[0024] The concentration of prepared ALA+chlorfenuron solution is 0.1mg / L+0.05mg / L(T1), 0.5mg / L+0.1mg / L(T2), 1.0mg / L+0.2mg / L(T3), 2.0mg / L+0.5mg / L (T4), 5.0mg / L+1.0mg / L (T5) and 10.0mg / L+2.0mg / L (T6), with clear water as the control (CK).
[0025] Leymus chinensis (Trin.) Tzvel.) seeds were placed in a beaker containing 50 mL of the above-prepared solution, placed in an incubator at 25° C., and treated for 24 hours. After the above treatment time is up, take out the seeds of each treatment and rinse them with tap water for 5 minutes, soak them in 10% sodium hypochlorite for 20 minutes, then rinse them with distilled water for 3 to 4 times, then place the seeds on filter paper, and let them dry naturally at room temperature for 48 hours. 50 seeds per treatment, repeated 3 times. The treated Leymus chinensis seeds were germinated in the light incubator at 15°C / 25°C (15°C / 12h dark, 25°C / 12h light) conditions. Add distilled water regularly and quantitatively every day to keep th...
Embodiment 2
[0031]Select Leymus chinensis seeds with plump grains, uniform size and no pests and diseases, rinse with distilled water after disinfection with sodium hypochlorite solution, and evenly spread them in a culture tray with double-layer filter paper to germinate. After one week, transplant the Leymus chinensis seedlings with consistent growth into pots. When Leymus chinensis seedlings grow to 18-20cm, spray ALA + forchlorfenuron solution on the roots of the pots and pots, the concentrations are 10mg / L+0.5mg / L (T7), 20mg / L+1.0mg / L (T8) , 60mg / L+2.5mg / L(T9), 100mg / L+5.0mg / L(T10), 150mg / L+7.5mg / L(T11), and 200mg / L+10mg / L(T12), with Spray the same amount of water as the control (CK). 40 seedlings per treatment, repeated 3 times. Samples were taken after 14 days of treatment. The results are shown in Table 2.
[0032] Table 2 The effect of ALA + forchlorfenuron complex solution on the growth and photosynthetic pigments of Leymus chinensis
[0033]
[0034] Note: Different let...
Embodiment 3
[0037] Select Leymus chinensis seeds with plump grains, uniform size and no pests and diseases, rinse with distilled water after disinfection with sodium hypochlorite solution, and evenly spread them in a culture tray with double-layer filter paper to germinate. After one week, transplant the Leymus chinensis seedlings with consistent growth into pots. The normal water supply of the soil was used as the control (CK1), and the relative soil water content was 50% for drought stress treatment. When Leymus chinensis seedlings grow to 20-23cm, spray ALA + forchlorfenuron solution on the drought stress treated seedlings, the concentration is 5mg / L+0.2mg / L (T13), 10mg / L+0.5mg / L (T14) , 20mg / L+1.5mg / L(T15), 50mg / L+2.5mg / L(T16), 75mg / L+4.0mg / L(T17) and 100mg / L+5.0mg / L(T18), with Spray the same amount of clear water as the control (CK2). 30 seedlings per treatment, repeated 3 times. In order to avoid the influence of light on the treatment effect, the treatment time was in the evenin...
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