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Preparation method for biodegradable tissue engineering skin stent

A tissue-engineered skin and biodegradable technology, applied in medical science, prostheses, etc., can solve the problems of incomplete sterilization of raw materials, large source limitations, and poor quality uniformity, so as to speed up the recovery of skin tissue, improve wound healing, Mechanically good effect

Active Publication Date: 2018-02-23
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although natural collagen is widely recognized as the most ideal raw material rich in skin, the collagen currently used is animal-derived collagen, which has large source limitations, poor quality uniformity and preparation process due to differences in source animals. The toxicity of chemical residual reagents caused by the frequent use of acids and bases in medicine, and the hidden dangers of animal-derived viruses such as mad cow disease may be caused by incomplete sterilization of raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Preparation of tissue engineering skin scaffold material

[0025] Step 1: Weigh human-like collagen and dissolve it in 10ml double-distilled water to obtain a human-like collagen solution with a mass fraction of 10%, and add carboxylated chitosan, sodium hyaluronate and pore-forming agent NaCl to it, add The mass volume ratio concentration of the amount and the solution is respectively 2%, 0.5% and 2.5%, stirring and dissolving evenly;

[0026] Step 2: Add bio-enzyme cross-linking agent transglutaminase to the solution in step 1, the addition amount is 60U / g protein, transfer it into a square mold after stirring evenly, and put it in a refrigerator at 4°C for 24 hours for cross-linking; Refrigerator freezing and -80°C ultra-low temperature freezing treatment for 3 hours each, and vacuum freeze-drying to obtain the skin scaffold material containing salt;

[0027] Step 3: The saline-containing skin scaffold material freeze-dried in step 2 was soaked and was...

Embodiment 2

[0030] Embodiment 2: Preparation of tissue engineering skin scaffold material

[0031] Step 1: weigh human-like collagen and dissolve it in 10ml double-distilled water to obtain a human-like collagen solution with a mass fraction of 20%, and add carboxylated chitosan, sodium hyaluronate and pore-forming agent NaCl to it, add The mass volume ratio concentration of the amount and the solution is respectively 1%, 0.25% and 3%, stirring and dissolving evenly;

[0032] Step 2: Add bio-enzyme cross-linking agent transglutaminase to the solution in step 1, the addition amount is 50U / g protein, transfer it into a square mold after stirring evenly, and put it in a refrigerator at 25°C for 18 hours to cross-link; Refrigerator freezing and -80°C ultra-low temperature freezing treatment for 3 hours each, and vacuum freeze-drying to obtain the skin scaffold material containing salt;

[0033] Step 3: The saline-containing skin scaffold material freeze-dried in step 2 was soaked and washed ...

Embodiment 3

[0035] Embodiment 3: Preparation of tissue engineering skin scaffold material

[0036] Step 1: weigh human-like collagen and dissolve it in 10ml double-distilled water to obtain a human-like collagen solution with a mass fraction of 15%, and add carboxylated chitosan, sodium hyaluronate and pore-forming agent NaCl to it, add The mass volume ratio concentration of the amount and the solution is respectively 1.5%, 0.1% and 2.5%, stirring and dissolving evenly;

[0037]Step 2: Add bio-enzyme cross-linking agent transglutaminase to the solution in step 1, the addition amount is 20U / g protein, transfer it into a square mold after stirring evenly, and place it in a refrigerator at 37°C for 12 hours for cross-linking; Refrigerator freezing and -80°C ultra-low temperature freezing treatment for 3 hours each, and vacuum freeze-drying to obtain the skin scaffold material containing salt;

[0038] Step 3: The saline-containing skin scaffold material freeze-dried in step 2 was soaked and...

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Abstract

The invention discloses a preparation method for a biodegradable tissue engineering skin stent. The method comprises the following steps: adding carboxylation chitosan and sodium hyaluronate as auxiliary raw materials and adding NaCl as a pore-foaming agent into an aqueous solution of human collagen, stirring till fully dissolving all the added materials, adding glutamine transaminase as a bio-enzyme cross-linking agent for enzymatic cross-linking, and then refrigerating, performing vacuum freeze drying, removing salt and sterilizing by irradiating with Co60, thereby acquiring a degradable three-dimensional tissue engineering skin stent for repairing skin. The skin stent has high biocompatibility and excellent pore structure, is suitable for cell growth and can be used for treating full-thickness defect and scald.

Description

technical field [0001] The invention relates to a preparation method of a biodegradable tissue engineering skin support, belonging to the technical field of biomedical materials. Background technique [0002] Clinically, patients with burns and skin defects are in urgent need of skin transplantation. Autologous skin transplantation has the advantage of not causing immune rejection, but for patients with large area burns, the source is often absolutely insufficient, and the skin donor site may not be able to autologously. Healing, infection or scar formation and other pains and risks, so its application is subject to certain restrictions; xenogeneic or allogeneic skin transplantation has the advantages of sufficient quantity and relatively wide sources, but strong immune rejection will occur after skin transplantation, and the transplanted skin It is often rejected very quickly, and it also has limitations to a certain extent. Therefore, an artificial skin that can replace th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/60A61L27/58A61L27/54C08J9/26C08J3/24C08L89/00C08L5/08
CPCA61L27/48A61L27/54A61L27/58A61L27/60A61L2300/236A61L2300/252A61L2300/404A61L2300/412A61L2300/604C08J3/246C08J9/26C08J2201/0484C08J2389/00C08J2405/08C08L89/00C08L5/08
Inventor 范代娣朱晨辉惠俊峰米钰郑晓燕马沛雷桓
Owner NORTHWEST UNIV(CN)
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