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ELISA method for detecting titer of cLHRH antibody in chicken serum

A technology of antibody titer and chicken serum, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of wound infection, relatively high technical experience requirements for operators, and no related reports.

Active Publication Date: 2018-02-16
成都瑞盛高科技股份有限公司
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  • Abstract
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Problems solved by technology

However, in addition to being "cruel", surgical "castration" has major disadvantages: it will cause bleeding, easily cause wound infection, and may even lead to animal death; it is very stressful to animals, which will cause food loss and weight loss. The breeding cycle is extended; the technology is primitive and labor-intensive, which is not suitable for modern intensive and large-scale production; the requirements for the operator's technical experience are quite high, and the range of applicable animals is also very limited; "Break resistance" and increase the burden on livestock owners
It can be used to determine the titer of antibodies in ducks, geese, rats, Chinese bees, etc., but there is no relevant report on the titer of cLHRH antibodies in chicken serum

Method used

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  • ELISA method for detecting titer of cLHRH antibody in chicken serum
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  • ELISA method for detecting titer of cLHRH antibody in chicken serum

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Embodiment 1

[0044] 1. Reagents used

[0045] Coating antigen: cLHRH-Lys10-cLHRH, an artificially synthesized antigen polypeptide with a molecular weight of 3353.82, a purity of 90.82%, and a concentration of 1 mg / ml; the amino acid sequence is:

[0046] Glu-His-Trp-Ser-Tyr-Gly-Leu-Gln-Pro-Gly-Lys-Lys-Lys-Lys-Lys-Lys-Lys-Lys-Lys-Lys-Glu-His-Trp-Ser-Tyr- Gly-Leu-Gln-Pro-Gly.

[0047] Enzyme-labeled antibody: rabbit anti-chicken enzyme-labeled secondary antibody, purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.

[0048] Coating buffer: 0.1mol / L, pH=9.0 and pH=7.4 phosphate buffer.

[0049] Washing solution PBST: 0.01mol / L, pH7.4 phosphate buffered saline (PBS)+0.05% Tween-20.

[0050] Blocking solution: 0.1 mol / L, pH=9.0 phosphate buffer containing 2% gelatin + 0.05% Tween-20.

[0051] Compound Peptidase Inhibitor Cocktail: purchased from BIO-TOOL Company.

[0052] Sample diluent: 0.1 mol / L pH8.0 phosphate buffer containing 1% BSA+0.5% compound peptidase inhibitor cockta...

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Abstract

The invention provides an ELISA method for detecting the titer of a cLHRH antibody in chicken serum. The method comprises the following steps: preparation of a coating antigen, coating, washing, blocking, addition of a sample, addition of an enzyme-labeled antibody, color development, termination of a reaction, and titer determination. According to the invention, synthetic peptide containing dualepitopes and polylysine is designed as the coating antigen, and the ELISA method containing the coating antigen is established for the first time for determination of the titer of the cLHRH antibody in chicken serum. The coating antigen is cLHRH-Lys10-cLHRH; and the titer determination is implemented by using an end-point dilution method or a standard curve method. The method provided by the invention can be used for detecting the titer of the cLHRH antibody in chicken serum, and is accurate, reliable and stable in detection results.

Description

technical field [0001] The invention belongs to the technical field of measuring LHRH antibody titer, and in particular relates to an ELISA method for detecting cLHRH antibody titer in chicken serum. Background technique [0002] "Castration" is one of the major inventions in ancient my country, commonly known as "castration". It is a traditional animal husbandry production technology to improve meat quality. It has been used for thousands of years and has a decisive position in my country's animal husbandry economy and traditional food civilization. In addition to improving the meat quality, taste and flavor of livestock and poultry, castration can make animals docile, easy to manage, use, gain weight quickly, reduce calorie consumption, and improve feed utilization. However, in addition to being "cruel", surgical "castration" has major disadvantages: it will cause bleeding, easily cause wound infection, and may even lead to animal death; it is very stressful to animals, wh...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/543
Inventor 吉传义何信群杨建波龚文波李峰谢建勇伏刚方鹏飞严成郝伟伟潘华柱扶海
Owner 成都瑞盛高科技股份有限公司
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