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System and method for separating and purifying flavones and polysaccharide from oldenlandia diffusa

A technology for separation and purification of Hedyotis diffusa, applied in the field of separation of effective components of traditional Chinese medicine, can solve the problems of long process time, complicated operation, cumbersome process, etc., and achieve the effect of simple process, high separation precision and good impurity removal rate

Active Publication Date: 2018-02-16
XIAMEN FILTER & MEMBRANE TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] Patent Publication No. CN101496845A provides an extract of Hedyotis diffusa and its separation and preparation method, extracting and separating four substances such as cartilinic acid and cartilin in Hedyotis, and extracting them by water extraction After decompression evaporation to make the extract, alcohol precipitation, filtration, re-concentration, re-alcohol precipitation and centrifuge centrifugation, and finally enter the 3000-6000D ultrafiltration membrane system system, and then enter the macroporous adsorbent and industrial chromatographic separation system. This process has the characteristics of high product components, but the disadvantage of this method is that the extraction process uses highly dangerous ethanol, and in order to obtain a clear filtrate, the pretreatment process needs to go through multiple centrifugation, alcohol precipitation, standing and other processes, especially When using adsorbents to separate flavonoids, the resin column must be disconnected after all the filtrate has passed through, and then perform operations such as water washing and alcohol analysis. The overall process takes a long time and the process is cumbersome and complicated. The production cycle from extraction and preparation to final Hedyotis diffusa extract product is as long as 10 days”
[0005] Patent Publication No. CN104173526A discloses a preparation method of Hedyotis diffusa flavonoids, which adopts the method of vacuum evaporation concentration and polyamide resin column chromatography separation and purification. The active ingredients are not processed, which is a great waste. In the separation and purification process of polyamide resin column chromatography, it is also necessary to wait for all the filtrate to pass through before the resin can be cleaned and analyzed. The overall process takes a long time and the process is cumbersome. complex
[0006] There are no relevant reports on the method of separating and purifying flavonoids and polysaccharides from Hedyotis diffusa by combining membrane separation technology and continuous ion exchange technology

Method used

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  • System and method for separating and purifying flavones and polysaccharide from oldenlandia diffusa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Weigh 5 kg of pulverized Hedyotis grass powder, add 60 kg of water for ultrasonic extraction for 2 hours, and remove large particles by coarse filtration to obtain 58 L of filtrate;

[0041]Step 2: The filtrate enters the ceramic membrane equipment for clarification and removal of impurities. The membrane area is 0.24 square meters, the pore size is 200nm, the operating pressure is 2.5bar, the operating temperature is controlled within 60°C, and the continuous operation is 540min. During the process, the concentrated solution is washed with water, and the average membrane flux About 51LMH (liter / square meter per hour), get 110L ceramic membrane dialysate;

[0042] Step 3: The ceramic membrane dialysate enters the ultrafiltration membrane equipment for the concentration and separation of polysaccharides. It is equipped with an ultrafiltration roll-type membrane element with a molecular weight cut-off of 2500 Daltons and a specification of 2540. The operating press...

Embodiment 2

[0045] Step 1: Weigh 12 kg of crushed Hedyotis grass powder, add 150 kg of water for ultrasonic extraction for 2 hours, and remove large particles by coarse filtration to obtain 141 L of filtrate;

[0046] Step 2: The filtrate enters the ceramic membrane equipment for clarification and removal of impurities. The membrane area is 0.24 square meters, the pore size is 500nm, the operating pressure is 3.6bar, the operating temperature is controlled within 60°C, and the continuous operation is 1130min. During the process, the concentrate is washed with water, and the average membrane flux About 62LMH (liter / square meter per hour), get 280L ceramic membrane dialysate;

[0047] Step 3: The ceramic membrane dialysate enters the ultrafiltration membrane equipment for the concentration and separation of polysaccharides, the piped membrane, the roll-type membrane core with a molecular weight cut-off of 5000 Daltons, the operating pressure is 12bar, the operating temperature is controlled ...

Embodiment 3

[0050] Step 1: Weigh 54 kg of pulverized Hedyotis grass powder, add 650 kg of water for ultrasonic extraction for 2.5 hours, and remove large particles by coarse filtration to obtain 610 L of filtrate;

[0051] Step 2: The filtrate enters the ceramic membrane equipment for clarification and removal of impurities. The membrane area is 0.48 square meters, the pore size is 100nm, the operating pressure is 3.6bar, the operating temperature is controlled within 60°C, and the continuous operation is 1080min. During the process, the concentrated solution is washed with water, and the average membrane flux About 69LMH (liter / square meter per hour), get 1220L ceramic membrane dialysate;

[0052] Step 3: The ceramic membrane dialysate enters the ultrafiltration membrane equipment for the concentration and separation of polysaccharides, the piped membrane, the roll-type membrane core with a molecular weight cut-off of 10,000 Daltons, the operating pressure is 8bar, the operating temperatu...

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Abstract

The invention discloses a system for separating and purifying flavones and polysaccharide from oldenlandia diffusa. The system is characterized by comprising an ultrasonic extraction device, a pre-filtering device and ceramic membrane equipment which are connected in sequence, wherein a dialyzate outlet of the ceramic membrane equipment is connected with ultrafiltration membrane equipment; a concentrated liquid outlet of the ultrafiltration membrane equipment is connected with a first vacuum concentration and spraying device; the dialyzate outlet is connected with a continuous ion exchange andspectrum separation device; the continuous ion exchange and spectrum separation device and the dialyzate outlet are sequentially connected with membrane concentration equipment and a second vacuum concentration device and spraying device. The invention further discloses a method for separating and purifying flavones and polysaccharide from oldenlandia diffusa. The system is simple in process, capable of achieving separation and purification of flavones and polysaccharide from oldenlandia diffusa rapidly at a time, short in production cycle, possible in continuous material feeding and discharge, stable in operation, high in product content, full-automatic in operation, capable of saving a great amount of labor and materials and applicable to industrial popularization.

Description

technical field [0001] The invention relates to the technical field of separation of effective components of traditional Chinese medicine, in particular to a system and method for separating and purifying flavonoids and polysaccharides of Hedyotis diffusa by using membrane separation and continuous ion exchange technology. Background technique [0002] Hedyotis diffusa is an annual scattered herb with bitter, sweet and cold nature. It has the functions of clearing away heat, promoting dampness and detoxification. It is used to treat dysentery, urethritis, jaundice, appendicitis, sores, swelling and pain, and mosquito bites. It is a traditional Chinese medicine prescription. One of the commonly used drugs in China. Modern phytochemical studies have shown that Hedyotis diffusa contains a variety of chemical components, including flavonoids, anthraquinones, sterols, polysaccharides, terpenoids and aliphatic compounds. In clinical treatment, Hedyotis diffusa is widely used, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07D311/30C07D311/40
CPCC07D311/30C07D311/40C08B37/0003Y02P20/10
Inventor 虞美辉彭广生湛科葛尚勇范仁锋
Owner XIAMEN FILTER & MEMBRANE TECH
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