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High-density euglena culture method

A technology for high-density culture and euglena, which is applied in the field of euglena preparation, can solve the problems of slow growth of euglena, inability to become a dominant species, unfavorable scale production, etc. The effect of the production cycle

Inactive Publication Date: 2018-02-13
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the growth rate of euglena in the autotrophic state is slow, the production cycle is long, and the production cost is high, which is not conducive to large-scale production; while in the heterotrophic culture, the growth rate of euglena is slower than that of bacteria, and it cannot become the dominant species.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The high-density cultivation method of euglena of the present invention comprises the following steps:

[0026] The first step is to expand the algae species. Prepare the euglena concomitant culture medium in a 5.0L Erlenmeyer flask, and sterilize it in an autoclave at 121°C for 20 minutes. Then inoculate the algal species in the seed bottle into the above-mentioned sterilized and cooled medium, and the inoculation amount of the euglena algae species expansion is 10%. Shake the flask every 4 hours for the expansion of algae, and irradiate with an artificial LED light source at a light intensity of 2500 lx, and cultivate for 3 days. The formula of euglena adjunct culture medium contains: 1.0g of peptone, 0.9g of yeast powder, 0.05g of potassium dihydrogen phosphate, 0.1g of magnesium sulfate, 0.06g of calcium chloride, 1mL of Fe-EDTA mother solution, and trace amount in each liter of medium. Element mother solution 1mL and vitamin mother solution 1mL, control pH to 6; ...

Embodiment 2

[0031] The difference from Example 1 is that in the second step, an artificial LED light source is used to irradiate with a light intensity of 3000lx; the aeration ratio is 1:1v / vm, ​​the carbon dioxide content accounts for 1%, and the culture is cultivated for 3 days.

[0032] According to biochemical testing, the concentration of euglena cells prepared in this example is 2.4 million / mL.

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PUM

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Abstract

The invention discloses a high-density euglena culture method and belongs to the technical field of euglena preparation. The preparation method comprises the steps: firstly, performing expanding culture of euglena alga filaments, wherein an euglena mixotrophic culture medium required by production is utilized as an expanding culture medium; then inoculating expanding cultured euglena alga filaments into a plastic film photobioreactor, wherein the euglena mixotrophic culture medium is added by stages, the inoculum size in each stage is about 30%, and an artificial LED light source is utilized for culture. By means of a mixotrophic culture mode, on one hand, the high-density euglena culture method disclosed by the invention can utilize organisms in the culture medium to obtain rapid proliferation of euglena; on the other hand, under illumination and ventilation culture conditions, CO2 is fixed to the maximum extent, and the chlorophyll content of the euglena individual is improved. The cell concentration of the euglena obtained by the method disclosed by the invention can reach 3 millions / Ml, and the dry cell weight of the euglena is obviously improved.

Description

technical field [0001] The invention relates to the technical field of euglena preparation, in particular to a high-density culture method for euglena. Background technique [0002] Euglena is an intermediate organism between animals and plants. It has the protein composition of animals and plants and is a good bait organism; 50% of the dry weight of Euglena is protein, and its quality is better than that produced by plants. Protein, containing 20 kinds of amino acids, including 8 kinds of essential amino acids such as isoleucine, is more suitable as food than chlorella. In 2013, my country approved euglena as a new food raw material, which makes euglena have great Good market prospects. In recent years, Japan and other countries have successively developed patented products of euglena as fish bait. my country started late in this field, and the related technology is not yet mature. Exploring the high-density cultivation of euglena will help realize the production of euglena ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李悦明周文礼许丽娜张文慧夏修峦徐建春范栋
Owner QINGDAO KEHAI BIOLOGICAL
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