Dentin-pulp complex preparation method

Abstract

The invention provides a dentin-pulp complex preparation method. The preparation method comprises the following steps: 1) after informed consent of a patient, collecting caries-free premolar teeth pulled out for orthodontic treatment in surgery departments of stomatological hospitals, removing soft tissues, washing clean and storing in -80 DEG C refrigerator for standby application; 2) in preparation, taking out a sample tooth, cutting a crown enamel part out on a low-speed hard tissue slicing machine and leaving and taking a dentin layer from the top of a pulp cavity to the crown, wherein thethickness is 1.5mm; 3) continuing cutting the obtained dentin into dentin slices with length * width * height as 2mm * 2mm * 2mm; 4) soaking dentin chips into in a 5 to 20%w / w EDTA solution for 10min, washing by PBS for 5min three times, soaking for 1min in a 19%w / w citric acid solution and washing by PBS for 5min three times. The dentin-pulp complex preparation method disclosed by the inventionhas the following beneficial effects: 1) a standardized preparation method can improve repeatability and scientificity of correlational researches; 2) the standardized preparation method can be used for detecting odotogenetic differentiation potential of seed cells in an experiment.

Description

technical field [0001] The invention relates to a preparation method of a tissue engineering complex, in particular to the preparation of a tissue engineering complex related to pulp regeneration, specifically a preparation method of a dentin-pulp complex. Background technique [0002] With the rapid development and wide application of tissue engineering technology, scaffold materials have also been improved and updated to varying degrees. In dental pulp tissue engineering, the clinical application of tissue engineered dental pulp can become more realistic by using porous scaffolds that can maintain the survival of stem cells and regenerate blood vessels and nerves. Because the scaffold can not only replace the basic mechanical and biological functions of the tissue, but also contain growth factors, cytokines and even DNA fragments that help stem cells proliferate and differentiate, thereby controlling and guiding the target tissue to develop better and faster. In teeth wit...

AI Technical Summary

Problems solved by technology

However, it is difficult for these scaffold materials to form a suitable pore ratio and pore size to facilitate the entry and diffusion of cells and nutrients, as well as the removal of metabolic substances.

Moreover, the dentin pulp-like structure formed in vivo cannot realize the orderly arrangement of odontoblast layer, predentin layer and mature dentin layer.

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