Method for cultivating detoxified mint
A cultivation method and mint technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of reduced economic output, slow variety renewal, and degradation of excellent traits, and achieve improved color, lush leaves, and environmental adaptability Improved effect
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Embodiment 1
[0015] A kind of detoxification mint cultivation method, comprises the following steps:
[0016] (1) Detoxify the poisonous female mint: treat the poisonous female mint at a temperature of 30°C and a relative air humidity of 80% for 3 hours, then take 2 cm of the top section of the poisonous female mint, cut Remove 1 piece of leaf, then soak in 80% ethanol solution for 80s, then use ultrasonic treatment for 15s, then take it out, use γ-rays to irradiate for 5s, and then put it in 80% ethanol solution Soak in water for 30s, then take it out, and then cut off the tip 0.5mm of the top section of the poisonous female mint for tissue culture;
[0017] (2) Tissue culture: Tissue culture the tip 0.5 mm of the top section of the poisonous female mint in step (1) with MS medium, the culture conditions are as follows: temperature is 22°C, light intensity is 1200 lux, and the light intensity is not less than 12 hours per day. Hour; The MS medium contains quercetin of its quality 0.0012%...
Embodiment 2
[0024] A kind of detoxification mint cultivation method, comprises the following steps:
[0025] (1) Detoxify the poisonous female mint: treat the poisonous female mint at a temperature of 35°C and a relative air humidity of 80% for 5 hours, then take 2 cm of the top section of the poisonous female mint, cut Remove 2 leaves, then soak them in 80% ethanol solution for 100s, then use ultrasonic treatment for 20s, then take them out, irradiate them with γ-rays for 8s, and then put them in 80% ethanol solution Soak in water for 40s, then take it out, and intercept the tip 0.5mm of the top section of the poisonous female mint for tissue culture;
[0026] (2) Tissue culture: Tissue culture the tip 0.5 mm of the top section of the poisonous female mint in step (1) with MS medium, the culture conditions are temperature at 24°C, light intensity at 1500 lux, and light at least 12 hours per day. Hour; The MS medium contains quercetin of its quality 0.0012%;
[0027] (3) Rooting culture...
Embodiment 3
[0033] A kind of detoxification mint cultivation method, comprises the following steps:
[0034] (1) Detoxify the poisonous female mint: treat the poisonous female mint for 4 hours at a temperature of 32°C and an air relative humidity of 80%, then take 2 cm of the top section of the poisonous female mint, cut Remove 2 leaves, then soak them in 80% ethanol solution for 90s, then use ultrasonic treatment for 18s, then take them out, irradiate them with γ-rays for 6s, and then put them in 80% ethanol solution Soak in water for 35s, then take it out, and cut off the tip 0.5mm of the top section of the poisonous female mint for tissue culture;
[0035] (2) Tissue culture: Tissue culture the tip 0.5 mm of the top section of the poisonous female mint in step (1) with MS medium, the culture conditions are temperature at 23°C, light intensity at 1300 lux, and light at least 12 hours per day. Hour; The MS medium contains quercetin of its quality 0.0012%;
[0036](3) Rooting culture: w...
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