Method for refining beta-thymidine in fermentation broth through ultrafiltration and ion exchange technology

Abstract

The invention discloses a method for refining beta-thymidine in a fermentation broth through an ultrafiltration and ion exchange technology. The method comprises (1) microfiltration of a fermentationbroth: treating the beta-thymidine fermentation broth through a filter film with film pore sizes of 50-1000nm, (2) ion exchange of the clean filtrate: treating the clean filtrate through cation and anion exchange columns to remove multivalent ions in the filtrate, (3) ultrafiltration of the ion exchange solution: treating the ion exchange solution through a filter film with molecular weight cut-off of 500-5000, and (4) chromatography concentration crystallization of the clean filtrate. The two-step film separation technology simplifies the existing multi-stage film separation process and extraction and separation processes are avoided. The ion exchange technology is used between two film separation processes so that the problem that a film separation rate is reduced because of concentration multiple improvement in the film separation process and metal salt precipitation in the practical production is solved. The method has the advantages of simple and reasonable processes, high extraction and separation degree, high yield and simple processes.

Description

technical field [0001] The invention relates to the technology in the field of production of β-thymidine, in particular to a method for refining β-thymidine in fermentation broth by ultrafiltration and ion exchange technology. Background technique [0002] β-thymidine, as a component of the genetic material deoxythymidine nucleic acid (DNA), exists in all biological cells and plays an important role in physiology. [0003] β-thymidine is the key intermediate for the synthesis of anti-AIDS antiviral drugs zidovudine and lamivudine in the pharmaceutical industry. One is to use 2-deoxy-N-phenylpentosamine as the starting material through chemical methods Synthesis of β-thymidine; one is to use Escherichia coli to ferment and produce β-thymidine with glucose and ammonia water as raw materials. However, due to the complex process of producing β-thymidine by chemical method, high requirements for production conditions, low product yield and high production cost, a series of defec...

AI Technical Summary

Problems solved by technology

However, in this method, the β-thymidine fermentation liquid has passed through four times of membrane filtration according to the removal accuracy, and the operation steps are cumbersome and time-consuming, which affects the product yield.

And because the fermentation broth contains a large amount of metal ions, as the concentration factor increases during the membrane filtration process, metal salts will continue to be precipitated from the clear liquid, which will adhere to the surface of the membrane core during the membrane filtration process, resulting in subsequent membrane filtration. The filtration speed is reduced, and even the membrane pores are blocked, which greatly affects the filtration effect

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