Factory-like plant tissue culture method and culture box implementing same

Abstract

The invention relates to a factory-like plant tissue culture method and a culture box implementing the method. The plant tissue culture method comprises the following steps: placing tissue culture vessels inside the culture boxes, and then laminating the culture boxes filled with the tissue culture vessels inside a culture room. The culture box implementing the method comprises a box body, an LEDlamp belt arranged on the upper edge of the inner side of the box body, a light reflecting assembly, a power supply assembly and a power line, wherein the box body is a plastic basket with more than 30% ventilating area; the light reflecting assembly is arranged in the center of the box body; the tissue culture vessels waiting for culture are placed inside the box body along the periphery of the light reflecting assembly; and the culture boxes filled with the tissue culture vessels are laminated inside the culture room. The culture box is simple and compact in structure, the space utilizationrate of the culture room is high, the culture box is applicable for factory-like production, relative to a tissue culture rack mode adopted in factories at present, the factory cost is reduced, the operating man-hour is reduced, and the listing cost of tissue culture seedlings is reduced.

Description

Technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for plant tissue culture for factory production. [0002] The invention also relates to a culture device, especially a plant tissue culture box used for factory production. Background technique [0003] Plant tissue culture is a method for rapid and mass propagation of plant seedlings indoors. Plant tissue culture, also known as plant cloning, refers to inoculating any organ, tissue or cell of a plant on an artificially configured culture medium containing nutrients and plant growth regulators through aseptic operations, and in vitro in an artificially controlled environment Cultivation, the process of making it grow and differentiate to form a complete plant. [0004] At present, tissue culture racks are mostly used in plant tissue culture operations of factory production, and the mainstream design is mostly the use of universal angle steel sprayed plastic racks, the outer ...

AI Technical Summary

Problems solved by technology

However, with the popularization of LED technology, special LED cold light source plant tissue culture lamps or LED tissue culture lamps with specific wavelengths have appeared, replacing conventional fluorescent tube lamps, reducing the heat dissipation pressure of the tissue culture environment, but due to the continued use of In the past bed frame mode, the space utilization rate was low. At the same time, in the case of large-scale production of tissue culture seedlings, this bed frame mode also highlights the problem of high overall cost of tissue culture plants.

On the other hand, in order to utilize the upper bed frame, the operator needs to climb up and down, place or remove the tissue culture bottles one by one, which is very inconvenient, so that the upper bed frame is often vacant

The bed frame of the cultivation room is fixed, and there are many dead corners, which also cause a lot of inconvenience to the environment of the cultivation room.

[0005] Some Chinese patents such as application numbers CN200920147137.4, CN201520227384.0, CN201620267196.5, CN201620680227.X all relate to a special incubator for plant tissue culture. This incubator has a number of miniaturized cultivation spaces with independent functions. It does not depend on the indoor environment where it is located, but its idea is still to meet the needs of laboratory work, rather than factory production, which does not help solve the problems highlighted by the factory bed frame model

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