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A kind of algae mixed cultivation method

A technology of mixed culture and algae, applied in the direction of single-cell algae, microbe-based methods, biochemical equipment and methods, etc., can solve the problems such as difficult harvesting of benthic diatoms, increase diversity, efficiently purify wastewater, harvest Receive convenient effect

Active Publication Date: 2019-11-12
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the method also solves the problem that benthic diatoms are not easy to harvest in the prior art

Method used

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  • A kind of algae mixed cultivation method
  • A kind of algae mixed cultivation method
  • A kind of algae mixed cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Mixed culture of Clerodes hardyrella and Nitzschia

[0050] 1) Temporary culture of Cladophyllum hardy and expanded cultivation of Nitzkiria

[0051] For temporary cultivation of hardy algae, put the healthy growth and bright green hardy algae with a wet weight of 5kg-5.5kg into a glass tank filled with 10L of sterilized seawater, at a room temperature of 15°C-20°C, and a salinity of 31‰- 33‰, natural light 5000Lx-7000Lx, aerated culture, absorb the bottom and change seawater 2L-3L every day, and use it after 2 days of temporary cultivation.

[0052] For the expanded cultivation of Nishiki, inoculate 10% of the algae into the f / 2 medium containing 25-30mg / L sodium silicate for cultivation. During the cultivation process, the room temperature is 15°C-20°C, the light is 4000Lx-5000Lx, and the salinity is 31‰ -33‰, pH 7.5-8.5, aerated for 10 days to reach a density of 10 5 cell / mL, the algae sludge was collected by centrifugation.

[0053] 2) Vaccination

[0054] Put 0...

Embodiment 2

[0061] Mixed culture of Cladoides and Navicula

[0062] 1) Temporary culture of Cladoella and expanded cultivation of Navicula

[0063] Temporary cultivation of Cladoides, 0.5kg-1kg (wet weight) of healthy growth and bright green Cladoides in a beaker filled with 1L of sterilized seawater, room temperature 15°C-18°C, salinity 30‰, LED white light intensity 4000Lx -6000Lx, change 0.5L of sterilized seawater every day, and inflate for 2-3 days.

[0064] For the expanded cultivation of Navicula, inoculate 10% of the algae species into the f / 2 medium containing 23-25mg / L sodium silicate and cultivate them. Temperature 30‰, pH 7.5-8.0, aerated culture density up to 10 5 cell / mL or more, collect the algae sludge by centrifugation.

[0065] 2) Vaccination

[0066] Put 500g (wet weight) of Cladophora temporarily raised in step 1) into a 4L sterilized seawater culture tank, add 20-25mg / L sodium nitrate and 2-3mg / L sodium dihydrogen phosphate, and inoculate after 1-2 days of aerated...

Embodiment 3

[0072] Mixed culture of water cotton (Spirogyra) and double brow algae

[0073] 1) Temporary cultivation of Spirogyra and expanded cultivation of Amphibious algae

[0074] Temporary cultivation of Spirogyra, temporary cultivation of Spirogyra 2kg-3kg (wet weight) in a glass tank with 5L of sterilized fresh water, room temperature 25°C, daylight intensity 8000Lx-10000Lx, 1 / 4-1 / 3 of the water for disinfection and cultivation every day, Inflate for 1-2 days.

[0075] For the expanded cultivation of Amphibious algae, inoculate 10% of the algae into the f / 2 medium containing 10mg / L sodium silicate for cultivation, the cultivation temperature is 20°C-25°C, the LED light intensity is 5000Lx-8000Lx, and the aerated culture density reaches 10 4 cell / mL, the algae sludge was collected by centrifugation.

[0076] 2) Vaccination

[0077] Put 1kg (wet weight) of Spirogyra temporarily raised in step 1 into a 5L sterilized freshwater culture tank, add 10-20mg / L urea, 4-5mg / L disodium hydr...

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Abstract

The invention provides a mixed culture method of alga and in particular relates to a mixed culture method of conferva and benthic diatoms. By adopting the method provided by the invention, a culture space is sufficiently utilized according to biological properties of the two types of alga; the culture method is more ecological and environment-friendly; the problem in a process of culturing the benthic diatoms on a carrier is avoided and the biomass of the benthic diatoms is extremely improved, so that high-density culture of the two types of alga is realized. Meanwhile, the problem in the prior art that the benthic diatoms are not easy to harvest is also solved through the method.

Description

technical field [0001] The patent relates to a mixed culture method of algae, in particular to a mixed culture method of filamentous green algae and benthic diatoms, belonging to the field of algae biotechnology. Background technique [0002] There are many kinds of diatoms, widely distributed, rich in various nutrients, including high unsaturated fatty acids (EPA, DHA), polysaccharides, proteins, etc. Cultivate bait. [0003] In particular, benthic diatoms are the necessary inducers and important bait for the attachment and metamorphosis of planktonic larvae during the cultivation of valuable marine economic animals such as abalone, sea urchin, and sea cucumber that grow prostrate. [0004] The quality and biomass of benthic diatoms are one of the key factors for the successful breeding of marine economic animals, which affect the growth rate and survival rate of seedlings. Moreover, as a fresh bait, diatoms also have the functions of regulating water quality, controlling...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 邢荣莲邵逸文马伟伟
Owner YANTAI UNIV
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