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Method for culturing microorganisms

A microbial culture and microorganism technology, applied in fungi and other directions, can solve the problems of loss of medium and difficult to control product temperature, and achieve the effect of increasing air exchange, increasing culture volume, and solving difficult product temperature control.

Inactive Publication Date: 2011-01-12
FOSHAN HAITIAN GAOMING FLAVORING & FOOD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For above-mentioned defect, the technical problem that the present invention solves is to provide a kind of culture method of microorganism, solves the problem such as difficult to control of the product temperature that exists in the little single culture vessel of laboratory condition culture microorganism in a large number of culture vessels, anoxic, culture medium moisture loses rapidly etc., and Make the quality of the strains produced reach the quality level of pure strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Aspergillus oryzae is subcultured and expanded, and the cultivation process of natural ventilation is as follows:

[0029] Wheat bran: polysaccharides: flour in a ratio of 6:2:2 to 8:1:1, and then add 80 to 130% water of the weight of the above ingredients, fully stir and mix to prepare a sporulation medium. The culture medium is divided into the above-mentioned incubators, and the thickness of the material is about 1-4 cm. The wells of the incubator are tightly plugged with sterile filter membranes or rubber stoppers. After the subpackaged medium container is sterilized by routine high temperature, turn the culture container back and forth while it is still hot to shake up the medium inside. Place the inoculation port of the incubator close to the flame and use an inoculation shovel to insert the pure species of Aspergillus oryzae that needs to be subcultured and expanded into the medium, and turn the culture container back and forth to allow the medium to fully mix w...

Embodiment 2

[0031] Aspergillus oryzae is subcultured and expanded, and the cultivation process of forced ventilation is as follows:

[0032]Wheat bran: waste residue after treatment of leguminous plants: flour in a ratio of 6:2:2 to 8:1:1, add water of 80% to 130% of the weight of the above ingredients, fully stir and mix to prepare a cultured base. The culture medium is divided into culture containers, and the thickness of the material is 3cm-8cm. The wells of the incubator are tightly plugged with sterile filter membranes or rubber stoppers. After the subpackaged culture container is sterilized at high temperature, turn the culture container back and forth while it is still hot to shake up the culture medium inside. Place the inoculation port of the culture container close to the flame and use an inoculation shovel to insert the pure species of Aspergillus oryzae that needs to be subcultured and expanded into the medium, and turn the culture container back and forth to allow the mediu...

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Abstract

The invention discloses a method for culturing microorganisms. The method comprises the following steps of: (1) preparing a culture medium for culturing pure microorganisms and putting the culture medium in a culture container for pure culture, wherein the culture container is a porous culture container; an inoculation port is separated from a scavenging port; and the bottom area of the culture container is multiple times that of the conventional culture container; (2) sterilizing at a high temperature and cooling by the conventional method and inoculating activated pure microorganisms for expanding; and (3) after inoculation is finished, uniformly mixing a strain and the culture medium and putting the mixture into a room for pure expansion and culturing. Due to the adoption of the method for culturing the microorganisms provided by the invention, the problems of difficult temperature control, oxygen deficiency, rapid water dispersion of the culture medium and the like existing in mass culturing of microorganisms in a small single culture container under a laboratory condition and the quality of a produced strain reaches the quality level of a pure strain.

Description

technical field [0001] The present invention relates to the field of microorganism cultivation, in particular to a method for cultivating microorganisms. Background technique [0002] Most of the industrial microorganisms used to produce fermented food are fungal microorganisms. To cultivate fungal microorganisms, a solid-state fermentation process is usually used. Traditional fungal purebred culture technology mainly uses containers such as test tubes or glass bottles as culture carriers. Because the bottom area of ​​this kind of culture container is small, the specific surface area of ​​the koji material in contact with the environment is larger, the heat transfer is faster, and the koji material can quickly reach the set temperature, which is convenient for product temperature management. But, there are the following deficiencies in such culture method, cause can not carry out the purebred culture of carrying out microorganism concentrated in a large number: [0003] 1....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 赵丽云洪钦辉吴梦弦黄文彪
Owner FOSHAN HAITIAN GAOMING FLAVORING & FOOD
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