Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Psychrobacter aquimaris and method for preparing flocculating agent by psychrobacter aquimaris

A flocculant, marine technology, applied in the field of water treatment, can solve the problem of low flocculation activity, achieve strong flocculation effect, promote settlement, and reduce the use of ethanol

Active Publication Date: 2018-01-23
ZHEJIANG OCEAN UNIV
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low flocculation activity in the preparation of extracellular polysaccharide flocculants by microorganisms at the present stage, the purpose of the present invention is to provide a strain of marine Pyrobacterium that can secrete exopolysaccharides with flocculation. It is separated and purified from sludge liquid, and the flocculant prepared from the exopolysaccharide secreted by the marine Pyrobacterium strain has high flocculation activity, strong flocculation ability and stable performance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Psychrobacter aquimaris and method for preparing flocculating agent by psychrobacter aquimaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing flocculant with marine cryobacterium strain GHF2, comprising the following steps:

[0032] (1) Inoculate the strain on a solid medium and culture it at 18°C ​​for 36 hours, then add sterile water to make a strain solution, and the volume ratio of sterile water to solid medium is 1.0:1;

[0033] (2) Inoculate the seed liquid into the liquid culture medium, the inoculation concentration is 0.8mL of the seed liquid / 100mL of the liquid medium, ferment and cultivate at 18°C ​​for 3 days, and the shaker speed is 160r / min, to prepare the fermentation liquid;

[0034] (3) Add the flocculation carrier to the fermented bacterial liquid, the addition is 5g / 100mL fermented bacterial liquid, then continue to ferment and cultivate for 1 day to obtain the raw material liquid, the flocculated carrier is sandy soil, preferably the natural sandy soil of cultivating Philippine clams, and the flocculated carrier is passed through 200 Sterilized after being sieved thr...

Embodiment 2

[0038] A method for preparing flocculant with marine cryobacterium strain GHF2, comprising the following steps:

[0039] (1) Inoculate the strain on the solid medium and cultivate it at 20°C for 42 hours, then add sterile water to make the strain liquid, the volume ratio of the sterile water to the solid medium is 1.25:1;

[0040] (2) Inoculate the seed liquid into the liquid medium, the inoculation concentration is 1.1mL of the seed liquid / 100mL of the liquid medium, ferment and cultivate at 20°C for 4 days, and the shaker speed is 180r / min, to prepare the fermented bacterial liquid;

[0041](3) Add the flocculation carrier in the fermented bacterial liquid, the addition is 7.5g / 100mL fermented bacterial liquid, then continue to ferment and cultivate for 2 days to obtain the raw material liquid, the flocculated carrier is sandy soil, preferably the natural sandy soil of cultivating Philippine clams, and the flocculated carrier is passed through Sieve through a 250-mesh sieve ...

Embodiment 3

[0045] (1) Inoculate the strain on a solid medium for 48 hours at 23°C, then add sterile water to make a strain solution, the volume ratio of sterile water to solid medium is 1.5:1;

[0046] (2) Inoculate the seed liquid into the liquid culture medium at an inoculation concentration of 1.4 mL of the seed liquid / 100 mL of liquid medium, ferment and cultivate at 23° C. for 5 days, and the shaker speed is 200 r / min to prepare the fermented bacterial liquid;

[0047] (3) Add the flocculation carrier in the fermented bacterial liquid, the addition is 10g / 100mL fermented bacterial liquid, then continue to ferment and cultivate for 3 days to obtain the raw material liquid, the flocculated carrier is sandy soil, preferably the natural sandy soil of cultivating Philippine clams, and the flocculated carrier is passed through 300 Sterilized after being sieved through a mesh sieve;

[0048] (4) Add ethanol to the raw material solution and let it settle, the volume ratio of ethanol to raw ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of water treatment, in particular to psychrobacter aquimaris and a method for preparing a flocculating agent by the psychrobacter aquimaris. The psychrobacter aquimaris Psychrobacter sp. GHF2 is separated and purified from sludge liquor spit by ruditapes philippinarum, a strain of the psychrobacter aquimaris is subjected to enlarging cultivation to prepare strain liquid, the strain liquid is fermented and cultivated to prepare fermented liquid, a flocculating carrier is added into the fermented liquid in a fermentation late period, and the flocculating carrier and added ethyl alcohol are mutually reinforced, so that extracellular polysaccharide in the fermented liquid is safely settled. The extracellular polysaccharide secreted by the screened psychrobacter aquimaris Psychrobacter sp. GHF2 has strong flocculating effects, the flocculating carrier is added in the fermentation late period, so that bacterial cells are clumped on the flocculating carrier, settling of the extracellular polysaccharide is promoted, use of the ethyl alcohol is reduced, and the obtained flocculating agent is strong in flocculating capacity and stable in performance.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a strain of Pyrobacterium marine and a method for preparing a flocculant using it. Background technique [0002] my country's urbanization has developed rapidly in the past ten years, but due to the lag in investment in sewage treatment facilities, low sewage treatment efficiency, and the discharge of a large amount of industrial wastewater and domestic sewage, the serious water environment has deteriorated, and the content of heavy metals, pigments, and suspended particles in the water body exceeds the standard. Water resources have now become an important factor restricting the development of social urbanization and industrialization. Improving sewage treatment technology and strengthening urban sewage treatment capacity has become a top priority. Among them, introducing flocculent particles of appropriate particle size into the sewage, using its huge surface area to fl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/01
Inventor 穆军崔霞杨桥
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com