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Method for tissue culture and rapid propagation of phyllanthus reticulatus poir

A tissue culture and red fish eye technology, which is applied in the field of plant tissue culture, can solve unseen problems and achieve the effects of simple operation, guaranteed proliferation coefficient, and robust plants.

Inactive Publication Date: 2018-01-19
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no research report related to tissue culture and other reproduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A red fish eye tissue culture rapid propagation method, comprising the steps of:

[0031] Step 1. Selection and disinfection of explants: Take the newly germinated shoots of healthy plants with red fish eyes, soak the shoots in 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 15-30 minutes, add 2-3 Drop / 100ml Tween-20 0.1% mercuric liter disinfection for 8-10min, rinse with sterile water 3-5 times, and finally remove surface moisture with sterile filter paper to obtain explants;

[0032] Step 2. Acquisition of sterile test-tube plantlets: place the explants obtained in step 1 in a clean bench, cut a 1.0-1.5cm long stem segment with an axillary bud with a scalpel, and inoculate it into the MS induction medium , cultured in the culture room for 30 days to obtain sterile test-tube plantlets, wherein the MS induction medium is: MS+0.5mg / L6-BA+0.1.0mg / LNAA+30g / L sucrose+5g / L agar, the pH value is 5.8 ;

[0033] Step 3, proliferation cult...

Embodiment 2

[0039] A red fish eye tissue culture rapid propagation method, comprising the steps of:

[0040] Step 1. Selection and disinfection of explants: Take the newly germinated shoots of healthy plants with red fish eyes, soak the shoots in 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 15-30 minutes, add 2-3 Drop / 100ml Tween-20 0.1% mercuric liter disinfection for 8-10min, rinse with sterile water 3-5 times, and finally remove surface moisture with sterile filter paper to obtain explants;

[0041] Step 2. Acquisition of sterile test-tube plantlets: place the explants obtained in step 1 in a clean bench, cut a 1.0-1.5cm long stem segment with an axillary bud with a scalpel, and inoculate it into the MS induction medium , cultured in a culture room for 30 days to obtain sterile test-tube plantlets, wherein the MS induction medium is: MS+1.0mg / L6-BA+0.2mg / LNAA+30g / L sucrose+5g / L agar, the pH value is 5.8;

[0042] Step 3, proliferation culture: I...

Embodiment 3

[0049] A red fish eye tissue culture rapid propagation method, comprising the steps of:

[0050] Step 1. Selection and disinfection of explants: Take the newly germinated shoots of healthy plants with red fish eyes, soak the shoots in 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 15-30 minutes, add 2-3 Drop / 100ml Tween-20 0.1% mercuric liter disinfection for 8-10min, rinse with sterile water 3-5 times, and finally remove surface moisture with sterile filter paper to obtain explants;

[0051] Step 2. Acquisition of sterile test-tube plantlets: place the explants obtained in step 1 in a clean bench, cut a 1.0-1.5cm long stem segment with an axillary bud with a scalpel, and inoculate it into the MS induction medium , cultured in a culture room for 30 days to obtain sterile test-tube plantlets, wherein the MS induction medium is: MS+1.5mg / L6-BA+0.5mg / LNAA+30g / L sucrose+5g / L agar, the pH value is 5.8;

[0052] Step 3, proliferation culture: i...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of phyllanthus reticulatus poir. The method comprises the following steps: selecting and disinfecting an explant; acquiring asterile test-tube plantlet; carrying out enrichment culture, carrying out strong seedling culture; carrying out rooting culture; and carrying out hardening-seedling and transplanting. The method has the advantages of being simple in culture substrate formula, simple and convenient in culture process, simple in operation, high in propagation coefficient, good in germchit quality and the like, and provides technical support for industrial seedling culture of phyllanthus reticulatus poir.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture. More specifically, the present invention relates to a method for rapid propagation of red fish eye tissue culture. Background technique [0002] Red fish eye (Phyllanthus reticulatus Poir), also known as longan, rotten head bowl, etc., is an erect or climbing shrub of Euphorbiaceae and Phyllanthus genus distributed in southern China such as Guangdong and Guangxi. Red fish eye has a long history of use in Guangxi Zhuang people. It is an important medicinal material in Guangxi Zhuang people. It has been recorded in "Guangxi Zhuang Autonomous Region Zhuang Medicine Quality Standard" (2008 edition). Efficacy, mainly for the treatment of rheumatic arthritis pain, bruises and other arthritic syndromes, and has various pharmacological effects in the treatment of diabetic diarrhea, analgesic anti-inflammatory, anti-hepatitis virus, antibacterial in vitro, anti-oxidation, etc., and has no ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李刚周琼王晓峰李竞才徐函兵唐美琼李莹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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