Building method of angelica dahurica tissue culture system
A method of establishment, the technology of Angelica dahurica, applied in the field of plant tissue culture, can solve the problems of high cost, low germination rate, and difficult germination, and achieve the effect of large market sales, large reproduction coefficient, and high seedling rate
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Embodiment 1
[0016] (1) Disinfection of explants: Rinse the collected stems of Angelica dahurica with clear water for 10 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution for 9 seconds in an ultra-clean workbench, and then wash them with sterile water 3 times, then rinse with 5% Antifoam solution for 4 minutes, rinse 3 times with sterile water, and then use sterile filter paper to absorb the water and set aside.
[0017] (2) Induction culture: cut the stems of Angelica dahurica sterilized in step (1) into 1.4cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate them into the induction culture Bud induction culture was carried out in the medium. After inoculation, they were first cultured in total darkness at 24°C for 2 days, and then placed in light for 9 hours a day with a light intensity of 1900 lx, and cultured at a temperature of 24°C until ad...
Embodiment 2
[0022] (1) Disinfection of explants: the collected stems of Angelica dahurica were rinsed with clean water for 11 minutes, and the impurities on them were gently brushed off with a brush, disinfected with 76% ethanol solution in an ultra-clean workbench for 19 seconds, and then washed with sterile water 4 times, then rinse with 5% Antifoam solution for 7 minutes, rinse with sterile water for 5 times, and then use sterile filter paper to absorb the water before use.
[0023] (2) Induction culture: cut the stems of Angelica dahurica sterilized in step (1) into 1.4cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate them into the induction culture Bud induction culture was carried out in the medium. After inoculation, they were first cultured in total darkness at 27°C for 3 days, and then placed in light for 11 hours a day with a light intensity of 2400 lx, and cultured at a temperature of 27...
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