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Blood type detection quality control product, preparation method thereof, and application thereof to blood type detection

A quality control product and blood type technology, applied in the field of blood transfusion testing, can solve problems such as wrong judgment results, and achieve the effect of maintaining biological performance, excellent anti-corrosion system, and good anti-corrosion ability

Inactive Publication Date: 2018-01-12
北京乐普诊断科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In this field, it is desired to develop a method for quality control of the ABO blood typing test card and the antihuman globulin test card, so as to avoid errors in the determination results caused by the problem of the reagent card itself during the use of the reagent card occur

Method used

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  • Blood type detection quality control product, preparation method thereof, and application thereof to blood type detection
  • Blood type detection quality control product, preparation method thereof, and application thereof to blood type detection
  • Blood type detection quality control product, preparation method thereof, and application thereof to blood type detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Step 1. Preparation of erythrocyte preservation solution

[0056] Add Hepes 15g / L, NaCl 9g / L to 1L of ultrapure water, mix well, adjust the pH to 7.0±0.1 with NaOH, add D-glucose 20g / L, adenine 0.1g / L, inosine 1g / L, Sodium citrate 5g / L, chloramphenicol 0.1g / L, neomycin 0.2g / L, gentamicin 1g / L, mix well, and filter with 0.22μm microporous membrane.

[0057] Step 2, preparation of erythrocyte reagent

[0058] Take A respectively 1 Type RhD(+), B-type RhD(+), O-type RhD(+), AB-type RhD(+), RhD(-) packed erythrocytes, dilute with the above erythrocyte preservation solution to a concentration of 0.8% erythrocyte reagent have to.

[0059] Step 3. Preparation of serum with different antibody types

[0060] A serum: add anti-B to AB serum to make anti-B titer>128;

[0061] B serum: Add anti-A to AB serum to make the anti-A titer > 128;

[0062] O serum: add anti-A and anti-B to AB serum, make anti-A titer>128, anti-B titer>128;

[0063] AB serum containing IgG type anti-...

Embodiment 2

[0083] A. the using method of described ABO, RhD blood group detection quality control product (micro-column gel method):

[0084] 1. ABO, RhD blood group detection quality control (micro-column gel method) includes a total of 6 bottles of reagents: A 1 RhD(+) red blood cells (like

[0085] Ben 1), Type B RhD(+) red blood cells (sample 2), A serum (sample 3), B serum (sample 4), O type RhD(+) red blood cells (sample 5) and RhD(-) red blood cells (sample 6 ) each vial;

[0086] 2. The specific operation steps of ABO and RhD blood group detection quality control products (micro-column gel method) are as follows:

[0087] (1) Take 4 microcolumn gel cards to be tested. Add sample 1 to wells 1 to 4 of the first card, 1 drop (50 μl) each; add sample 3 to wells Ac and Bc of the first microcolumn gel card, add 1 drop (50 μl) each, and place them in the marked wells respectively. Add 0.8% A 1 1 drop (50 μl) each of B standard red blood cells.

[0088] (2) Add sample 2 to wells 1 ...

Embodiment 3

[0116] In this embodiment, the quality control effect of the quality control product is verified

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Abstract

The invention provides a blood type detection quality control product, a preparation method thereof, and application thereof to blood type detection. The blood type detection quality control product comprises 6 bottles of ABO, an RhD blood type detection quality control product, 5 bottles of cross-matching blood quality control products and 4 bottles of irregular antibody sieving test quality control products. The blood type detection quality control product can guarantee accuracy, specificity, affinity and stability of blood type detection, avoids influence on the detection result caused by unqualified detection in the detection process, and creates conditions for accuracy of ABO blood type determination, cross-matching blood and irregular antibody screening.

Description

technical field [0001] The invention belongs to the technical field of blood transfusion testing, and relates to a blood type detection quality control product, a preparation method thereof and an application in blood type detection. Background technique [0002] The ABO blood group system is the first blood group system discovered by humans, and it is also one of the most clinically important blood group systems. [0003] Clinical blood transfusion is an important medical method for diagnosing and treating diseases and saving lives in modern medicine. At the same time, blood transfusion may cause a variety of adverse reactions, directly endangering the life of the patient, and irreparable mistakes may occur if there is a slight carelessness. In order to ensure the safety of blood transfusion, it is very important to choose reliable experimental methods and adopt experimental techniques with high specificity and sensitivity. [0004] As early as 1907, Hoktoen first propose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 杨洋魏明明陈江莎王占伟邱笑违
Owner 北京乐普诊断科技股份有限公司
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