Bionic water-permeable film and preparation method thereof
A water-permeable membrane and aquaporin technology, applied in semi-permeable membrane separation, chemical instruments and methods, membrane technology, etc., to achieve the effect of simple and easy operation and stable properties
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Embodiment 1
[0029] 1) Disperse 0.3 mg of aquaporin Z in 10 ml of 0.2 M / L calcium hydroxide nanofiber aqueous solution, and through ultrasonication and vibration, the aquaporin Z is evenly adsorbed to the surface of the nanofiber.
[0030] 2) Immerse the polysulfone membrane prepared by the phase inversion method into the dopamine Tris-HCl buffer solution with a concentration of 2 mg / ml, and react for 2 hours at room temperature with a constant temperature oscillator, and the pH value of the solution is 8.5 to obtain a porous dopamine-modified polysulfone membrane. support layer. After the reaction, the surface of the membrane was washed with ethanol and water, respectively.
[0031] 3) Suction filtration of the solution containing aquaporin onto the surface of the dopamine-modified polysulfone porous support layer, and further cross-linking with 10% glutaraldehyde aqueous solution for 1 h.
[0032] 4) Soak the obtained product in step 3) in 10 mM HCl for 1 h to remove the calcium hydroxi...
Embodiment 2
[0035] 1) Prepare 2mM cadmium nitrate aqueous solution, stir and quickly add 0.8mM NaOH aqueous solution until white precipitation of cadmium hydroxide appears in the system. After centrifugation and washing with water, the above-mentioned white precipitate is redispersed in deionized water to obtain an aqueous solution of cadmium hydroxide nanofibers.
[0036] 2) Disperse 0.5 mg of aquaporin 1 in 50 ml of 0.2 M / L cadmium hydroxide nanofiber aqueous solution, and through ultrasonication and vibration, aquaporin is evenly adsorbed to the surface of the cadmium hydroxide nanofiber.
[0037] 3) Suction filter the solution containing aquaporin onto the surface of the polycarbonate porous support layer, further cross-link with a 10% glutaraldehyde aqueous solution for 1 h, and then soak in 10 mM HCl for 1 h to remove the copper hydroxide nanofibers to obtain water-containing channels Biomimetic water-permeable membrane of protein 1.
[0038] To measure the performance of the biomi...
Embodiment 3
[0040] 1) Disperse 0.3 mg of aquaporin 4 in 50 ml of 0.2 M / L copper hydroxide nanofiber aqueous solution, and through ultrasonication and vibration, the aquaporin is evenly adsorbed to the surface of the nanofiber.
[0041] 2) Suction filter the solution containing aquaporin onto the surface of the alumina porous support layer, further cross-link with a 10% glutaraldehyde aqueous solution for 1 h, and then soak in 10 mM HCl for 1 h to remove the calcium hydroxide nanofibers to obtain the aquaporin 4 bionic permeable membrane.
[0042] Forward osmosis method was used to measure the performance of the water-permeable membrane. The supply liquid was deionized water, and the drawing liquid was 1M / L NaCl solution. The bionic water-permeable membrane containing aquaporin 4 in the above-mentioned embodiment was used for separation, and the water flux was 350Lm -2 h -1 , with a salt flux of 30gm -2 h -1 .
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