Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicine capable of controlling feline distemper as well as preparation method and applications of medicine

A technology of feline distemper and medicine, which is applied in the field of pet medicine, can solve the problems such as feline distemper cannot be cured, and achieve the effect of convenient long-distance transportation, broad application prospects, and potential harm of price reduction

Inactive Publication Date: 2018-01-12
北京京蒙细胞生物科技股份有限公司 +1
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that existing medicines cannot radically cure feline distemper, the invention provides a medicine for effectively controlling cat distemper, a preparation method of the medicine, and an application of the medicine in treating feline distemper disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine capable of controlling feline distemper as well as preparation method and applications of medicine
  • Medicine capable of controlling feline distemper as well as preparation method and applications of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, culture of human umbilical cord mesenchymal stem cells and collection of culture supernatant

[0030] 1. Preparation of human umbilical cord mesenchymal stem cells

[0031] The preparation of human umbilical cord mesenchymal stem cells was performed in an ultra-clean bench, and the following steps were used:

[0032] (1) Collect the umbilical cord tissue of a healthy person, and use normal saline to wash away the residual blood in the tissue and blood vessels;

[0033] (2) Separating and removing the umbilical cord vein, artery and amniotic tissue;

[0034] (3) Shred the gelatinous part of the umbilical cord tissue in step (2) to 1-2 mm 3 Tissue block, using IMDM medium (purchased from gibco) digestion solution containing 0.1% (M / V) collagenase I (purchased from sigma) and 0.25% trypsin (purchased from armesco) at 37 degrees, 100 rpm shaker digestion 2 hours;

[0035] (4) Centrifuge at 2500rpm for 10 minutes, remove the supernatant, and wash the precip...

Embodiment 2

[0040] Example 2, Collection of Bioactive Factors in Human Umbilical Cord Mesenchymal Stem Cell Culture Supernatant The steps of collecting bioactive factors in human umbilical cord mesenchymal stem cell culture supernatant include:

[0041] (1) Place the supernatant stored at -20°C collected in Example 1 at 4°C overnight to melt;

[0042] (2) Centrifuge at 2500rpm for 10 minutes, remove cell debris, and collect the culture supernatant of human umbilical cord mesenchymal stem cells;

[0043] (3) Use a stirring ultrafiltration cup (purchased from millipore) to carry out ultrafiltration. The ultrafiltration membrane molecular weight cut-off of the stirring ultrafiltration cup is 100Kda, and the human genome fragments, microorganisms and molecular weights exceeding 100Kda in the culture medium are removed. proteins and other substances; and

[0044] (4) The human umbilical cord mesenchymal stem cell culture supernatant after ultrafiltration is concentrated by ultrafiltration usi...

Embodiment 3

[0046] Embodiment 3, a kind of feline distemper medicine and preparation method thereof

[0047]A medicine for effectively controlling feline distemper, comprising the human umbilical cord mesenchymal stem cell culture supernatant containing bioactive factors collected in Example 2, recombinant feline ω-interferon, granulocyte colony-stimulating factor and water for injection. The molecular weight of the bioactive factor is 3-100Kda, the protein concentration of the bioactive factor in the human umbilical cord mesenchymal stem cell culture supernatant is 1-5 mg / mL, and the concentration of the recombinant feline ω-interferon is 5 mg / mL. ~200,000 U / mL, the concentration of the granulocyte colony-stimulating factor is 20-30 μg / mL. The optimum protein concentration of bioactive factors in the human umbilical cord mesenchymal stem cell culture supernatant is 2 mg / mL, the optimum concentration of the recombinant cat ω-interferon is 100,000 U / mL, and the granulocyte colony The conc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a medicine capable of controlling feline distemper as well as a preparation method and applications of the medicine. The medicine capable of controlling feline distemper comprises a culture supernatant of human umbilical cord mesenchymal stem cells containing bioactive factors, recombinant cat omega-interferon and granulocyte colony-stimulating factors, wherein the molecularweight of the bioactive factors is 3-100 Kda. The medicine can effectively control diseases caused by feline distemper viruses and alleviate the symptoms including lassitude, vomiting, decrease of leukocytes, and the like which are caused by virus infection. The medicine provided by the invention can be transported for a long distance and stored for a long term, is convenient to use, belongs to aspecific medicine aiming at feline panleukopenia virus, and has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of pet medical treatment, and relates to a medicine for controlling feline distemper and its preparation method and application. Background technique [0002] Feline distemper, also known as feline infectious enteritis and feline panleukopenia, is a highly contagious acute infectious disease caused by feline parvovirus, characterized by sudden high fever, vomiting, diarrhea, and a sharp decrease in white blood cells. [0003] Under natural conditions, it can be transmitted through direct contact and indirect contact with the virus. Cats of all ages can be infected. In most cases, cats under 1 year old are susceptible to infection. The infection rate can reach 70%, and the mortality rate is 50-60%. The mortality rate of kittens under 5 months of age is as high as 80-90%, and the incidence rate gradually decreases with age. After the cat is infected with feline parvovirus, a large amount of virus is contained...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/28A61K38/21C12N5/0775C12P21/00A61P31/20A61K38/19
Inventor 李志刚王煜骏丁炜王云虹万兴中田志海阮志王颖颖杜颖杨海莲吴兰兰刘洁白光伟聂晨飞马瑞
Owner 北京京蒙细胞生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com